中国药业2011，Vol.20Issue(14)：35-37,3.
液相色谱-串联质谱法测定柏子仁中黄曲霉毒素残留量
Detemination of Aflatoxin Residual Quantity in Platycladi Seed by LC-MS/MS
摘要
Abstract
Objective To establish a LC - MS/MS method for determining the residual quantity of aflatoxin B1, B2, G1 and G2 in platycladi seed. Methods The sample extraction solution was purified hy immune affinity column and eluted by methanol. The mobile phase was mehanol/acetonitrile -0. l mmol ammonium acetate. The detection was performed on the reversed phase chromatographic column Aglent Zorbax Extend - C18(250 mm ×4. 6 mm, 5 μm) by ESI - MS positive ion MRM mode. Results Aflatoxin G2 and B2 in the range of 0. 03 - 3.2 ng/mL and aflatoxin B1 and G1 in the range of 0. 1 - 10 ng/mL showed the good linear relationship. The limit of detection(LOD) was 0. 1 ng/mL and the limit of quantitation (LOQ) was 0. 2 ng/mL. The average recovery rates in three concentrations (0. 25, 1. 0, 5. 0 ng/mL) were 84. 1％ - 96. 8％, RSD 7.7％ - 13. 4％ . Conclusion This method is simple and rapid with strong specificity, high sensitivity and accurate results. which can be used to qualitative and quantitative determination of aflatoxin in platycladi seed.关键词
液相色谱-串联质谱法/柏子仁/黄曲霉毒素Key words
LC - MS/MS/ platycladi seed/ aflatoxin分类
医药卫生引用本文复制引用
杨晶,栾国华,杨润陆..液相色谱-串联质谱法测定柏子仁中黄曲霉毒素残留量[J].中国药业,2011,20(14):35-37,3.基金项目
国家重大新药创制创新药物研究开发技术平台项目,项目编号:2009ZX09308-006-7. （）
