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云南鬼灯檠属植物中岩白菜素含量比较

陈雯 孟珍贵 杨生超 陈建斌 李龙根 李萍萍 李晓波

安徽农业科学2011,Vol.39Issue(10):5767-5769,5772,4.
安徽农业科学2011,Vol.39Issue(10):5767-5769,5772,4.

云南鬼灯檠属植物中岩白菜素含量比较

Content Comparison of Bergenin in yunnan Genus Rodgersia

陈雯 1孟珍贵 1杨生超 1陈建斌 2李龙根 1李萍萍 1李晓波1

作者信息

  • 1. 云南农业大学农学与生物技术学院云南省中药材规范化种植技术指导中心,云南昆明,650201
  • 2. 国家农科专业基础实验教学示范中心,云南昆明,650201
  • 折叠

摘要

Abstract

[ Objective ] To compare the content of bergenin in the Yunnan genus Rodgersia with different species and growing areas. [Method]The content of bergenin in the Yunnan genus Rodgersia with different species and growing areas was determined by HPIC. The chromatographic conditions were as follows: column of Agilent Zorbax XDB-C18(4.6 mm ×l50 mm, 5 μm), mobile phase of acetonitrile(A) and 0.02% phosphoric acid solution ( B ), gradient elution ( 0-10 min,0% - 20% A, 100% - 80% B ), flow rate of 1 ml/min, column temperature of 30 ℃, sampling volume of 10 μl and detection wavelength of 275 nm. [ Result] Bergenin showed a good linear relationship with absorbance in the range of 3 to 48 μg/ml ( r=0. 999 6). Bergenin content was the highest in Rodgersia sambucifolia Hemsl. with 5.59%, and the lowest in Rodgersia aesculifolia Batalin with 3.45%. [ Conclusion ] Bergenin content in the genus Rodgersia was various in different species, and there were obvious differences among different populations from the same species.

关键词

鬼灯檠属(Rodgersia)/HPLC/岩白菜素/西南鬼灯檠(Rodgersia sambucifolia Hemsl.)/羽叶鬼灯檠(Rodgersiapinnata Franch.)/七叶鬼灯檠(Rodgersia aescullfolia Batalin)/光腹鬼灯檠(Rodgersia sambucifolia Hemsl.var.estrigosa J.T.Pan)

Key words

Rodgersia/ HPLC/ Bergenin/ Rodgersia sambucifolia Hemsl./ Rodgersia pinnata Franch./ Rodgersia aesculifolia Batalin/ Rodgersia sambucifolia Hemsl. var. estrigosa J. T. Pan

分类

农业科技

引用本文复制引用

陈雯,孟珍贵,杨生超,陈建斌,李龙根,李萍萍,李晓波..云南鬼灯檠属植物中岩白菜素含量比较[J].安徽农业科学,2011,39(10):5767-5769,5772,4.

基金项目

云南省科技厅重点产业创新工程(2008IF004). (2008IF004)

安徽农业科学

0517-6611

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