安徽农业科学2011,Vol.39Issue(10):5767-5769,5772,4.
云南鬼灯檠属植物中岩白菜素含量比较
Content Comparison of Bergenin in yunnan Genus Rodgersia
摘要
Abstract
[ Objective ] To compare the content of bergenin in the Yunnan genus Rodgersia with different species and growing areas. [Method]The content of bergenin in the Yunnan genus Rodgersia with different species and growing areas was determined by HPIC. The chromatographic conditions were as follows: column of Agilent Zorbax XDB-C18(4.6 mm ×l50 mm, 5 μm), mobile phase of acetonitrile(A) and 0.02% phosphoric acid solution ( B ), gradient elution ( 0-10 min,0% - 20% A, 100% - 80% B ), flow rate of 1 ml/min, column temperature of 30 ℃, sampling volume of 10 μl and detection wavelength of 275 nm. [ Result] Bergenin showed a good linear relationship with absorbance in the range of 3 to 48 μg/ml ( r=0. 999 6). Bergenin content was the highest in Rodgersia sambucifolia Hemsl. with 5.59%, and the lowest in Rodgersia aesculifolia Batalin with 3.45%. [ Conclusion ] Bergenin content in the genus Rodgersia was various in different species, and there were obvious differences among different populations from the same species.关键词
鬼灯檠属(Rodgersia)/HPLC/岩白菜素/西南鬼灯檠(Rodgersia sambucifolia Hemsl.)/羽叶鬼灯檠(Rodgersiapinnata Franch.)/七叶鬼灯檠(Rodgersia aescullfolia Batalin)/光腹鬼灯檠(Rodgersia sambucifolia Hemsl.var.estrigosa J.T.Pan)Key words
Rodgersia/ HPLC/ Bergenin/ Rodgersia sambucifolia Hemsl./ Rodgersia pinnata Franch./ Rodgersia aesculifolia Batalin/ Rodgersia sambucifolia Hemsl. var. estrigosa J. T. Pan分类
农业科技引用本文复制引用
陈雯,孟珍贵,杨生超,陈建斌,李龙根,李萍萍,李晓波..云南鬼灯檠属植物中岩白菜素含量比较[J].安徽农业科学,2011,39(10):5767-5769,5772,4.基金项目
云南省科技厅重点产业创新工程(2008IF004). (2008IF004)