重庆医学2011，Vol.40Issue(18)：1769-1771,封3,4.DOI:10.3969/j.issn.1671-8348.2011.18.001
异常黑胆质成熟剂总酚与顺铂、多西他赛联合对人宫颈癌SiHa细胞的影响
The effect of combination of total phenolics from abnormal Savda Munziq with cisplatin or docetaxel on human cervical cancer SiHa cells
摘要
Abstract
Objective To investigate the effects of combination of total phenolics from abnormal Savda Munziq water extracts with cisplatin or docetaxel on human cervical cancer SiHa cells in vitro ,and to explore the mechanism.Methods SiHa cells were treated with total phenolics combined with cisplatin or docetaxel.Inhibition of cellular proliferation was detected by MTT assay,cellular morphology was observed by inverted phase microscope,and levels of Bcl-2 and telomerase expression in SiHa cells were detected by flow cytometry.Results The inhibitory rates of cellular proliferation of total phenolics-cisplatin group and phenolics-docetaxel group were significantly higher than those in cisplatin group and docetaxel group(P＜0.05) , respectively.Cells became small and round , and a lot of detached , round and dead cell could be seen.Levels of cellular Bcl-2 , and telomerase expression were markedly decreased(P＜0.05).Conclusion Total phenolics from abnormal Savda Munziq combined with cisplatin or docetaxel can inhibit proliferation of SiHa cells via inhibiting cellular Bcl-2 and telomerase expression levels.关键词
基因,Bcl-2/端粒,末端转移酶/细胞增殖/细胞凋亡/异常黑胆质成熟剂Key words
genes , Bcl-2 : telornerase / cell proliferation/ apoptosis / abnormal Savda Munziq引用本文复制引用
王国宁,米开热木·麦麦提,古扎丽努尔·阿不力孜..异常黑胆质成熟剂总酚与顺铂、多西他赛联合对人宫颈癌SiHa细胞的影响[J].重庆医学,2011,40(18):1769-1771,封3,4.基金项目
国家自然科学基金资助项目(30860325). （30860325）
