外科理论与实践2011，Vol.16Issue(3)：248-251,4.
胃癌乳斑微转移在胃癌干细胞及前驱细胞研究中的意义
Significance of omental milky spots micrometastasis in screening gastric cancer stem cells and progenitor cells
曾亮 1胡祥 1张怡 2黄罡1
作者信息
- 1. 大连医科大学附属第一医院普通外科
- 2. 大连医科大学美容医学院,辽宁,大连,116011
- 折叠
摘要
Abstract
Objective To investigate significance of omental milky spot micrometastasis model in screening cancer stem cells and progenitor cells. Methods 1×104 MFC cells were injected intraperitoneal. After 72 h of intraperitoneal injection, mice were sacrificed and the omentum was excised and the gastric cancer cells in micrometastasis were observed. The cells were suspended and injected subcutaneously into the right and left flanks of NOD/SCID mice with anesthesia. After 16 weeks all mice were sacrificed, and the tumors were removed and analyzed by histology(HE、CK20and CEA). The cells in the micrometastasis were collected and immunocytochemical staining of the cancer stem cells was carried out as markers related to CD133,CD44,CD24,CD34, and CD324. Results Of the 40 mice (dose range: 1×101to 1×103) injected with the gastric cancer cells in micrometastasis, one mouse transplanted with 5×102 cells generated a tumor, and tumors were consistently generated after injection of 1 ×103 cells. In contrast, one tumor was generated after the injection of 5×104 cells in the control group, and tumors were consistently generated after the injection of 5×106 cells. The histology of the tumors, as expressed by HE staining, showed the same poor differentiated adenocarcinomas. The immunohistochemical markers CK20 and CEA reveal similar staining patterns in both groups. Gastric cancer cells in the micrometastasis were high in expressing CD133 and CD44, and low in expressing CD324. Conclusions Omental milky spots are a highly efficient “natural filter” for screening gastric cancer stem cells. Gastric cancer stem cells in the micrometastasis are high in expressing CD133 and CD44, and low in expressing CD324.关键词
胃癌/胃癌干细胞/乳斑Key words
Gastric cancer/ Gastric cancer stem cells/ Omental milky spots分类
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曾亮,胡祥,张怡,黄罡..胃癌乳斑微转移在胃癌干细胞及前驱细胞研究中的意义[J].外科理论与实践,2011,16(3):248-251,4.
