法医学杂志2011，Vol.27Issue(2)：107-111,5.DOI:10.3969/j.issn.1004-5619.2011.02.007
闭合性脑损伤后血清和海马的蛋白质表达
Expression of Proteins in Serum and Hippocampus after Closed Brain Injury in Rats
摘要
Abstract
Objective To explore the difference of expression of proteins between the serum and hippocampus after brain injury in rats. Methods Male SD rats were used to establish brain injury model.The changes of proteins expression profile in serum and hippocampus at different time after brain injury were analyzed using weak cationic exchanger (WCX2) chips and immobilized metal affinity capture arrays-Cu (IMAC-Cu) chips by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry. Results A total of 436 protein peaks were detected in serum and 346 protein peaks were detected in hippocampus using WCX2 chips. A total of 229 protein peaks were detected in serum and 345 protein peaks were detected in hippocampus using IMAC-Cu chips. The same 10 protein peaks were respectively detected in serum and hippocampus using WCX2 chips. The same 13 protein peaks were respectively detected in serum and hippocampus using IMAC-Cu chips. Conclusion The changes of protein expression profile in serum and hippocampus are obvious after closed brain injury and show a significant difference.The different proteins detected in serum and hippocampus using the same chip could be biochemical markers for determining brain injury.关键词
法医遗传学/脑损伤/血清/海马/蛋白质芯片/大鼠Key words
forensic genetics/ brain injury/ serum/ hippocampus/ protein chip/ rats分类
社会科学引用本文复制引用
舒清明,李志强,李灵芝,杨术旺,战丽,张永亮..闭合性脑损伤后血清和海马的蛋白质表达[J].法医学杂志,2011,27(2):107-111,5.基金项目
国家自然科学基金资助项目(30471934) （30471934）
