中国当代儿科杂志2011，Vol.13Issue(6)：495-498,4.
Wnt5a在肛门直肠畸形患儿末端直肠组织中的表达及意义
Expression of Wnt5a in the terminal rectum of children With anorectal malformation
摘要
Abstract
Objective To study the expression of Wnt5a protein in the terminal rectum of children with anorectal malformation (ARM) and the possible association between Wnt5a and ARM. Methods Specimens were obtained from 20 children with ARM, 7 children with acquired rectovestibular fistula and 6 children with non-gastrointestinal tract disease (control group). The expression of Wnt5a protein in the terminal rectum was determined by immunohistochemistry and Western blot. Results Wnt5a was mainly expressed in the rectum of the myenteric nerve plexus, mucosal layer and submucosa in the control group. Compared with the control group, Wnt5a expression in the terminal rectum decreased significantly in the ARM group, and decreased more significantly in children with high ARM. The results of Western blot showed the expression of Wnt5a protein in the high, intermediate and low ARM groups were significantly lower than that in the acquired rectovestibular fistula and the control groups (P ＜0.01 ). The expression of Wnt5a protein in the high and the intermediate ARM groups were also lower than that in the low ARM group (P ＜ 0.01 ). There was no significant difference in the Wnt5a protein expression between the acquired rectovestibular fistula and the control groups. Conclusions The expression of Wnt5a in the terminal rectum decreases in children with ARM, suggesting Wnt5a may play an important role in the development of ARM.关键词
Wnt5a/肛门直肠畸形/儿童Key words
Wnt5a/ Anorectal malformation/ Child分类
医药卫生引用本文复制引用
贾慧敏,陈青江,张涛,白玉作,王维林..Wnt5a在肛门直肠畸形患儿末端直肠组织中的表达及意义[J].中国当代儿科杂志,2011,13(6):495-498,4.基金项目
国家自然科学基金资助项目(项目批准号:30801199) （项目批准号:30801199）
辽宁省科技攻关计划(2007225005-3) （2007225005-3）
辽宁省教育厅重点实验室项目(LS2010171). （LS2010171）
