现代检验医学杂志2011，Vol.26Issue(3)：14-16,3.DOI:10.3969/j.issn.1671-7414.2011.03.005
间接免疫荧光法与化学发光法检测抗核抗体的对比分析
Comparison of Indirect Immunofluorescence Assay and CMIA for Detecting Antinuclear Antibodies
汪光蓉 1蔡艳娟 2王强 1凡瞿明 1毛明 1杜琴 1张国元1
作者信息
- 1. 川北医学院附属医院检验科,四川南充,637000
- 2. 川北医学院检验系,四川南充,637000
- 折叠
摘要
Abstract
Objective To compare indirect immunofluorescence assay (IIFA) and chemoluminescence assay(CMIA) for detecting antinuclear antibodies (ANA). Methods A total of 240 serum samples were obtained from patients with established autoimmune disease,and all samples were used for ANA detection by both IIFA and CMIA. In eases where discrepancy occurred in the results by the two methods ,extractable nuclear antigens were detected using immunoblotting. Results The positivity rate of ANA detected by IIFA and CMIA was significantly different ( 90. 83 ％ and 78. 75％ , respectively,P ＜0. 05). All of the samples with different ANA results from two methods were detected for ANA profile. Positive rate of ANA profile was 14. 63％ in 41 cases with IIFA positive and CMIA negative ,while 66. 67％ in 12 cases with IIFA negative and CMIA positive. The difference was significant (P＜0. 05). For uncommon patterns,the percent agreement of the two methods was lowered in ANA detection than common patterns. Conclusion IIFA was more sensitive than CMIA in detecting the ANA. IIFA prescreening combined with CMIA can obtain the information of the nuclear pattem and allow the observation of the titer alterations. The combination of two or more testing methods can greatly enhance the accuracy of the resultS.关键词
抗核抗体/化学发光法/间接免疫荧光法Key words
antinuclear antibodies /chemoluminescence assay /indirea immunofluo-rescent assay分类
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汪光蓉,蔡艳娟,王强,凡瞿明,毛明,杜琴,张国元..间接免疫荧光法与化学发光法检测抗核抗体的对比分析[J].现代检验医学杂志,2011,26(3):14-16,3.
