重庆医学2011，Vol.40Issue(18)：1820-1821,1825,3.DOI:10.3969/j.issn.1671-8348.2011.18.022
冷冻保护剂对不同质量精液顶体完整性的影响
Influence of cryoprotectant on acrosome integrity of different quality of sperm
摘要
Abstract
Objective To explore influence of cryoprotectant on acrosome integrity of different quality of sperm. Methods 500 semen samples from young volunteers were collected. Semen analysis was conducted according to the WHO recommended methods.They were divided into 6 groups: normal group, less sperm group, malformed sperm group, weak sperm group, less-weak spermgroup and less-weak-malformed sperm group. Cryoprotectant were prepared using glycerol, egg yolk and sodium citrate. Semen sampies were frozen slowly in liquid nitrogen and recovered after one month. Papanicolaou stain was employed and acrosome integrity was observed by light microscopy. Results Comparison of rates of acrosome integrity between groups with and without cryoprotectant after thawing, there was statistically significant difference (P ＜ 0. 05). Before freezing, it was also statistically significant difference when compared the rates of acrosome integrity in malformed sperm group,less-weak-malformed sperm group to those in other groups(P＜0.05). Conclusion Cryoprotectant can protect effectively the structure and function of sperm in the process of semen cryopreservationo关键词
精液保存/冷冻/顶体Key words
semen preservation/ freezing/ acrosome引用本文复制引用
张益,陈莹,计垣,李红,杨继高,孙大光,杨柳,黄静..冷冻保护剂对不同质量精液顶体完整性的影响[J].重庆医学,2011,40(18):1820-1821,1825,3.基金项目
重庆市人口和计划生育科学技术研究院自然科学基金资助项目(08-11). （08-11）
