现代检验医学杂志2011，Vol.26Issue(3)：28-29,64,3.DOI:10.3969/j.issn.1671-7414.2011.03.010
自噬相关基因Beclin-1的克隆及原核表达载体的构建
Cloning and Construction of Beclin-1 Prokaryotic Expression Vector
摘要
Abstract
Objective To clone and construct of Beclin-1 prokaryotic expression vector. Methods The primers were designed according to the coding region of Beclin-l DNA sequences. Total RNA was extracted from LNCaP cells and Beclin-1 was amplificated by PCR,then the amplified product was ligated into pQE-80 vector by endonuclease digestion and the recombinant vector was comfirmed by DNA sequencing. Results Beclin-l was amplified by RT-PCR and cloned into pQE-80 vector by the digestion of restriction endonuclease. The coding sequence of Beclin-l was comfirmed identical to that published in GenBank. Conclusion The pQE-80-Beclin-1 prokaryotic expression vector has been successfully constructed, which lay the basis for the further autophagy expriments.关键词
自噬/Beclin-1/反转录PCR/基因重组/pQE-80Key words
autophagy/Beclin-1/RT-PCR/gene recombination/pQE-80分类
生物科学引用本文复制引用
任楠楠,刘家云,马越云,杨静,苏明权,郝晓柯..自噬相关基因Beclin-1的克隆及原核表达载体的构建[J].现代检验医学杂志,2011,26(3):28-29,64,3.基金项目
国家自然科学基金(No.30973463). （No.30973463）
