现代检验医学杂志2011，Vol.26Issue(3)：30-34,5.DOI:10.3969/j.issn.1671-7414.2011.03.011
8种深部真菌DNA微阵列鉴定方法的建立及应用
Development of a Oligonucleotide Microarray for Detection of Eight Common Deep Fungi
摘要
Abstract
Objective To develop a oligonucleotide microarray for detection of eight common deep fungi. Method8 Eight clinical common deep fungi were choosen for our research,including Candida albicans ,Candida glabrata , Candida krusei,Candida Ntropicalis , Candida parapsilosis , Aspergillus terreus , Aspergillus flavus , Aspergillus funigatus and Rhizopus oryzae. The ITS region of fungi was amplified with a pair of universal primers and labled with biotin in the meantime. The probes were designed in the variable region of target DNA sequence and fixed on the nylon membrane. Our DNA microarray was validated by using 45 clinical isolates as blinded samples. Results Eight clinical common deep fungi were detected with our DNA microarray successfully within 8 hours. The probes for Candida albicans ,Candida glabrata ,Candida krusei ,Candida tropicalrs ,Candida parapsilosis ,Aspergillus terreus,Aspergillus flavus and Rhizopus oryzae had high specificity;The probes for Aspergillus funigatus had weak cross reaction with Aspergillus flavus ,but it didn't influence the results of identification. The identification results of 45 clinical isolates by using our DNA microarray were consistent with clinical results. Conclusion Membrane-based microarray coupled with ITS probes possessed sufficient power to identify clinical common deep fungi rapidly and accurately in 8 hours and had the prospect of clinical applications.关键词
常见深部真菌/DNA微阵列/核糖体RNA基因Key words
common deep fungi / DNA microarray / ribosomal RNA gene分类
医药卫生引用本文复制引用
胡一敏,周新,郑璇,谢焱,刘松梅,鲁双燕,黎四维..8种深部真菌DNA微阵列鉴定方法的建立及应用[J].现代检验医学杂志,2011,26(3):30-34,5.基金项目
国家自然基金资助项目,编号30672012. （）
