现代检验医学杂志2011，Vol.26Issue(3)：44-46,3.DOI:10.3969/j.issn.1671-7414.2011.03.016
5种血清miRNA的检测在前列腺癌诊断中的价值评估
Analyze the Serum Level of Five miRNAs in Prostate Cancer Patients
朱琳 1马越云 1范渝红 1岳乔红 1韦晓明 1郝晓柯1
作者信息
- 1. 第四军医大学西京医院检验科,西安,710032
- 折叠
摘要
Abstract
Objective To detect the serum level of miRNA-141,miRNA-16,miRNA-103,miRNA-574 and miRNA-34b in prostate cancer patients. Then evaluate the value of the miRNA markers in prostate cancer diagnosis. Methods Total RNA including miRNA was extracted from serum samples of normal people,benign prostatic hyperplasia (BPH) and prostate cancer (PCa) patients. The serum levels of the 5miRNA were detected using fluorescence real-time PCR. And the association between miRNA markers and cancer development was analyzed using GraphPad Prism software. Results The serum level of miRNA-103 and miRNA-34b were significantly up-regulated in BPH and PCa patients. Especially miRNA-34b in PCa increased average 2. 2 × 104 folds than in normal people,and correlated with high PSA. All the other 4 miRNA did not change a lot in BPH and PCa patients comparing with normal people. Conclusion miRNA-34b is a potential diagnostic serum marker of prostate cancer,might be equal to PSA.关键词
miRNA/前列腺癌/前列腺增生/血清Key words
miRNA /prostatic cancer (PCa) /benign prostatic hyperplasia (BPH) /serum分类
医药卫生引用本文复制引用
朱琳,马越云,范渝红,岳乔红,韦晓明,郝晓柯..5种血清miRNA的检测在前列腺癌诊断中的价值评估[J].现代检验医学杂志,2011,26(3):44-46,3.
