中国当代儿科杂志2011，Vol.13Issue(6)：514-517,4.
mitoKATP通道开放剂对高氧诱导人A549细胞凋亡的保护作用
Protective effects of mitochondrial ATP-sensitive potassium channel on A549 cell apoptosis induced by hyperoxia
邹新艳 1董文斌 1邹丹 1李清平 1雷小平 1翟雪松 1陈枫2
作者信息
- 1. 泸州医学院附属医院,新生儿科,四川,泸州,646000
- 2. 泸州医学院附属医院,医学实验研究中心,四川,泸州,646000
- 折叠
摘要
Abstract
Objective To explore the protective effects of mitochondrial ATP-sensitive potassium channel opener diazoxide on hyperoxia-induced apoptosis of type Ⅱ alveolar epithelial cells ( A549 cells ) and possible mechanisms.Methods A549 cells were cultured in vitro and divided randomly into control, hyperoxia and diazoxide group.The hyperoxia group was exposed to a mixture of O2 (900 mL/L) and CO2 (50 mL/L) for 10 minutes, then cultured in a closed environment.The diazoxide group was pretreated with diazoxide of 100 μmol/L for 24 hrs before hyperxia induction.The cells were collected 12, 24 and 48 hrs after culture.The morphologic changes of A549 cells were observed under an inverted microscope.A549 cell apoptosis was detected by flow cytometry.The expression of Omi/HtrA2 in the endochylema of A549 cells was determined by immunohistochemistry.Results A549 cells were damaged and the changes in morphology of the cells were serious in the hyperoxia group.The apoptosis rate of A549 cells and the expression of Omi/HtrA2 in the endochylema increased in the hyperoxia group compared with the control group ( P ＜ 0.05 ).The growth and the morphology of A549 cells were greatly improved and the cell injuries were obviously alleviated in the diazoxide group.The expression of Omi/HtrA2 in the endochylema and the apoptosis rate of A549 cells were significantly reduced in the diazoxide group compared with the hyperoxia group ( P ＜ 0.05 ).Conclusions Diazoxide as an opener of mitoKATP channel can reduce the expression of Omi/HtrA2 and the apoptosis rate of A549 cells, thus relieves the injury of A549 cells induced by hyperoxia.关键词
高氧/二氮嗪/线粒体ATP敏感钾通道/凋亡/Omi/HtrA2/A549细胞Key words
Hyperoxia/ Diazoxide/ Mitochondrial ATP-sensitive potassium channel/ Apotosis/ Omi/HtrA2/ A549 cell分类
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邹新艳,董文斌,邹丹,李清平,雷小平,翟雪松,陈枫..mitoKATP通道开放剂对高氧诱导人A549细胞凋亡的保护作用[J].中国当代儿科杂志,2011,13(6):514-517,4.
