安徽农业科学2011，Vol.39Issue(10)：5931-5932,2.
抗沙丁胺醇单克隆抗体制备与鉴定
Preparation and Identification of Monoclonal Antibodies against Salbutamol
摘要
Abstract
[Objective] To prepare specific monoclonal antibodies against salbutamol and thus to provide the basis for the immunological detection. [ Method] BALB/c mice were immunized with SAL-BSA. Hybridoma lines ( SAL mAb) secreting monoclonal antibody against SAL were established through cell fusion. Ascites of SAL mAb were prepared in vivo and the immunological characteristics were observed. [ Result ] Two hybridoma lines,2D9 and IC4,were established, and the indirect ELISA titers of the cell supernatant and ascites were 1:1 × 104 and 1:1 × 106 ,respectively. Their isotype was IgG1. The median inhibitory concentration ( IC50 ) of the SAL mAb against SAL was 1.14 ng/mL It had cress-reactivity of 100.00% against salbutamol and 26.09% against clenobuterol hydrochloride,and no cross-reaction was found between the SAL mAb and other compounds. [ Conclusion] The high-titer,sensitive and specific anti-SAL monoclonal antibodies have been produced,which makes it possible to develop immunoassay for detection of SAL residues.关键词
沙丁胺醇/杂交瘤/单克隆抗体Key words
Salbutamo/ Hybridomas/ Monoclonal antibody分类
农业科技引用本文复制引用
李春生,吴萌,李君华,齐颖颖,李玉静,闫静辉..抗沙丁胺醇单克隆抗体制备与鉴定[J].安徽农业科学,2011,39(10):5931-5932,2.基金项目
河北省科技支撑计划项目(09227134D) （09227134D）
石家庄市科技支撑计划项目(10150142A). （10150142A）
