安徽农业科学2011，Vol.39Issue(10)：5926-5928,5970,4.
饲料玉米中优质乳酸菌的分离和鉴定
Isolation and Identification of High-Quality Lactic Acid Bacteria in Forage Corn
摘要
Abstract
[ Objective ] To screen suitable lactic acid bacteria strains from forage com which can be used as silage additives. [ Method ] The lactic acid bacteria strains were isolated by inoculation on MRS solid medium containing calcium carbonate, and they were preliminarily identified through rnorphological,physiological and biochemical experiments. The acid production efficiency was determined. Twelve strains having better acid-production ability were selected,and their salt tolerance and acid tolerance were detected. The sequences of their 16 S rDNA were also analyzed. [Result]A total of 44 lactic acid bacteria strains were isolated from the forage corn. As evidenced by the physiological and biochemical experiments,the twelve strains having better acid-production ability belonged to Leuconostoc,Lactobacillus and Enterococcus, respectively,and they had strong salt tolerance and acid tolerance. According to the sequences of 16 S rD NA,A4, B9, B1 1, B12 and B14 were Lactobacillus plantarum;A1 ,A2,A7 ,Al 1 and B8 ,Leuconostoc mesenteroides dextran subspecies; and A8 and A9 ,Enterococcus hirae. [ Conclusion ] The lactic acid bacteria strains with better acid-production ability isolated from forage corn can be developed into silage additives.关键词
饲料玉米/乳酸菌/分离/鉴定/16S rDNAKey words
Forage corn/ Lactic acid bacteria/ Isolation/ Identification/ 16 S rDNA分类
农业科技引用本文复制引用
严萍,张永辉,麦热姆妮萨·艾麦尔,阿不都克尤木·买买提,乌斯满·依米提..饲料玉米中优质乳酸菌的分离和鉴定[J].安徽农业科学,2011,39(10):5926-5928,5970,4.基金项目
国家自然科学基金项目(3076008)资助. （3076008）
