中国组织工程研究与临床康复2011，Vol.15Issue(24)：4437-4439,3.DOI:10.3969/j.issn.1673-8225.2011.24.016
灯盏花素对腹膜透析液诱导人腹膜间皮细胞转化生长因子β1的影响
Effect of breviscapine on transforming growth factor beta 1 in human peritoneal mesothelial cells induced by peritoneal dialysis solution
摘要
Abstract
BACKGROUND: Based on the understanding of breviscapine effects and mechanism on inhibiting and delaying peritoneal failure, breviscapine is expected to be widely introduced to prolong peritoneal dialysis time, improve the quality of dialysis, and reduce dialysis failure rate so as to promote long-term effect of peritoneal dialysis in patients with end stage renal diseases. OBJECTIVE: To observe the effects of breviscapine on the secretion of transforming growth factor beta 1 (TGF-β1) and proliferation activity of human peritoneal mesothelial cells (HPMCs) cultured in vitro with peritoneal dialysis solution (PDS). METHODS: HPMCs were cultured in vitro and divided into five groups: control group, PDS group, 5 μmol/L breviscapine group, 10 μmol/L breviscapine group, and 20 μmol/L breviscapine group. The levels of TGF-β1 and proliferation of HPMCs in supernatant were detected and compared.RESULTS AND CONCLUSION: Significantly increased levels of TGF-β1 and decreased cell proliferation activity were observed in the PDS group. The level of TGF-β1 in the PDS group was higher than that in the 5 μmol/L breviscapine group (P ＜ 0.05), and significantly higher than that in the 10 μmol/L breviscapine group and 20 μmol/L breviscapine group (P ＜ 0.01). The proliferation activity in the PDS group was lower than that in the 5 μmol/L breviscapine group (P ＜ 0.05), and significantly lower than that in the 10 μmol/L breviscapine group and 20 μmol/L breviscapine group (P ＜ 0.01). The results indicated that breviscapine can reduce the secretion of TGF-β1 in cultured HPMCs. Also it can restore the reduced cell proliferation activity caused by PDS．关键词
腹膜透析/腹膜间皮细胞/灯盏花素/细胞增殖/转化生长因子β1分类
医药卫生引用本文复制引用
杜飞,张克非,高原,张文辉,袁立英..灯盏花素对腹膜透析液诱导人腹膜间皮细胞转化生长因子β1的影响[J].中国组织工程研究与临床康复,2011,15(24):4437-4439,3.基金项目
贵州省卫生厅优秀医学青年人才基金(No.gzwkj2009-2-009). （No.gzwkj2009-2-009）
