中国组织工程研究与临床康复2011，Vol.15Issue(24)：4440-4443,4.DOI:10.3969/j.issn.1673-8225.2011.24.017
不同吸氧浓度对新生鼠视网膜血管发育和血管内皮生长因子表达的影响
Oxygen concentration variation influences the severity of retinopathy and vascular endothelial growth factor expression in newborn rats
摘要
Abstract
BACKGROUND: Oxygen plays an important role in premature retinal neovascularization, but its scope of oxygen and the specific mechanism of retinopathy are not clear.OBJECTIVE: To study the effects of different concentrations of oxygen on neovascularization in oxygen-induced retinopathy of newborn rats.METHODS: Forty newly born rats were divided into four groups: air group (10 rats) was raised in air room. Fluctuation group 1, 2, 3 (10 rats) was exposed to cycles of 50％-20％, 40％-10％, 50％-10％ for 14 days, respectively. Retinas were dissected and stained by adenosine-diphosphatase (ADPase) histochemistry for assessment of intraretinal vascular development and preretinal angiogenic vessel growth. Expressions of vascular endothelial growth factor (VEGF) were studied by Western-blot.RESULTS AND CONCLUSION: Retina flat-mounts presented increasing neovascular tufts, severity of retinal neovascularization and the number of vascular nuclei extending from the internal limiting membrane into the vitreous, as well as VEGF-expression in fluctuation group 2 and fluctuation group 3. There were no obviously neovascularization in the air and fluctuation 1 groups. This study suggested that a small change in blood oxygen profile could trigger a disproportionate increase in subsequent neovascularization, accompanied by changes of retinal VEGF level. VEGF promote retinal neovascularization. The over-expression of VEGF plays a synergistic role during the formation of neovascularization. Partial pressure of inspiratory oxygen was positively correlated with the retinopathy. Hypoxia may be more important for retinal neovascularization on the occurrence of retinopathy than hyperoxia.关键词
早产儿视网膜病/新生血管/氧环境/血管内皮生长因子/大鼠/眼科组织工程分类
医药卫生引用本文复制引用
魏二霞,柯晓云,陈慧,刘子彬,吴伟..不同吸氧浓度对新生鼠视网膜血管发育和血管内皮生长因子表达的影响[J].中国组织工程研究与临床康复,2011,15(24):4440-4443,4.基金项目
广东省自然科学基金项目(7005196),PEDF 抑制早产儿视网病变新生血管的实验研究. （7005196）
