中国组织工程研究与临床康复2011，Vol.15Issue(24)：4461-4464,4.DOI:10.3969/j.issn.1673-8225.2011.24.022
高糖状态下小鼠肾小球足细胞凋亡和组成型光形态建成1蛋白的表达
High glucose induces podocyte apoptosis and constitutive photomorphogenic 1 expression changes
摘要
Abstract
BACKGROUND: Studies have shown that constitutive photomorphogenic 1 (COP1) is involved in cell apoptosis.OBJECTIVE: To observe the effects of high glucose exposure on expression of in vitro cultured podocyte apoptosis and COP1 expression.METHODS: Mouse podocytes of an immortalized cell line were treated with different concentrations of glucose. Apoptosis index and mortality index at different culture time were evaluated by flow cytometry to screen best dose-effect glucose concentration (30 mmol/L), which was used to treat high-glucose group. Control group and mannitol group (30 mmol/L) were set. RESULTS AND CONCLUSION: Glucose induced podocyte apoptosis and death in time- and dose-dependent manner in certain concentration (P ＜ 0.05). With increasing glucose concentration, mortality index was significantly elevated, but apoptosis index remained unchanged. Compared with control group, apoptosis index was significantly increased in high glucose group (P ＜ 0.05), but COP1 mRNA and protein expression was significantly decreased (P ＜ 0.05). Results showed that changes in COP1 expression may play a critical role in pathogenesis of high glucose induced podocyte apoptosis.关键词
肾小球足细胞/高糖/组成型光形态建成1蛋白/凋亡/组织构建分类
基础医学引用本文复制引用
王晓林,甘华,杜晓刚..高糖状态下小鼠肾小球足细胞凋亡和组成型光形态建成1蛋白的表达[J].中国组织工程研究与临床康复,2011,15(24):4461-4464,4.基金项目
课题受高等学校博士学科点专项科研基金(20070631008) 资助,S 抑制性COP1 介导的p53 泛素化降解防治糖尿病肾病大鼠肾小球足细胞凋亡. （20070631008）
