重庆医学2011，Vol.40Issue(19)：1881-1884,4.DOI:10.3969/j.issn.1671-8348.2011.19.004
过表达缝隙连接蛋白Cx43与人脑胶质瘤细胞U251侵袭性的研究
A study of connexin Cx43 overexpression on U251 glioma cell invasion
摘要
Abstract
Objective Through in vitro overexpression of connexin Cx43 on U251 glioma cell invasion studies.To provide a new therapeutic approach the inhibition of invasion of malignant tumor.Methods Connexin Cx43 by constructing eukaryotic expression plasmid and transfected into U251 cells overexpressing connexin Cx43 , RT-PCR and weston-blot detected the expression of Cx43mRNA levels.Traswell cell culture plates with MTT method and the invasive ability of U251 cells to change.Results 1.successfullyconstructed the recombinant plasmid pCMV-Cx43 cDNA 2.successfully transfected U251 cells and selected stably transfected cell lines overexpressing Cx43.3.TRASWELL cell culture plates with MTT assay after the overexpression of Cx43 U251 cell infiltration capacity than the control group decreased.Conclusion Over expression of connexin Cx43 can inhibit invasion of U251 glioma cells.关键词
质粒转染/缝隙连接蛋白Cx43/人脑胶质瘤细胞/侵袭性Key words
plasmid transfection/ gap junction protein Cx43 mRNA/ human glioma cells引用本文复制引用
闻海兵,邹树峰,李嘉杰,洪涛..过表达缝隙连接蛋白Cx43与人脑胶质瘤细胞U251侵袭性的研究[J].重庆医学,2011,40(19):1881-1884,4.基金项目
国家自然科学基金资助项目(30760286). （30760286）
