中德临床肿瘤学杂志（英文版）2011，Vol.10Issue(8)：462-467,6.DOI:10.1007/s10330-011-0823-6
对比分析黑斑息肉综合征合并大肠癌组织肿瘤基因
Analysis of differentially expressed tumor-related genes in Peutz-Jeghers syndrome combined with colorectal carcinoma with cDNA microarrays
摘要
Abstract
Objective: The aim of the study was to screen the differentially expressed genes of Peutz-Jeghers syndrome (PJS) and colorectal carcinoma (CRC). Methods: This study used cDNA microarray to comparatively analyze the gene expression profiles of 4 cases of PJS combined with colorectal adenocarcinoma vs. normal mucosae. The cDNA microarray contained 8064 human genes, and then using RT-PCR to test three genes of all. Results: The experimental data showed that fourteen genes were differentially expressed, which were up-regulated in PJS. Fifty-one genes expressions were altered in CRCs, of which 32 were up-regulated, as compared to the normal mucosae. In addition, 5 genes were similarly altered in both PJS and CRCs. RT-PCR analyses confirmed the cDNA microarray data for three of those genes: LATS2, APC and MADH4. Conclusion: LCN2, USP4, GRO3, HYAL1 and APC - these differentially expressed genes likely represent biomarkers for early detection of CRC and may be potential therapeutic targets.关键词
Peutz-Jeghers syndrome (PJS)/colorectal carcinoma (CRC)/cDNA microarrays/gene/analysisKey words
Peutz-Jeghers syndrome (PJS)/colorectal carcinoma (CRC)/cDNA microarrays/gene/analysis引用本文复制引用
Xiaosan Zhu,Qiaofang Sang,Xiaojuan Zhan ,Yichen Dai,Qingzhen Nan,Zhangxin Chen,Junpei Xie,Wei Zeng,Yuka Fu,Yuanyuan Lin,Qingna Lian..对比分析黑斑息肉综合征合并大肠癌组织肿瘤基因[J].中德临床肿瘤学杂志（英文版）,2011,10(8):462-467,6.基金项目
Supported by the grants from Funded Project based by the Health Innovation of the Xiamen Municipal Science and Technology Programme(No.3502Z20084031) and Medical Technology Innovation Project of Nanjing Military Region (No.09MA066). （No.3502Z20084031）
