中国组织工程研究与临床康复2011，Vol.15Issue(19)：3421-3426,6.DOI:10.3969/j.issn.1673-8225.2011.19.001
与软骨细胞共培养和条件培养液诱导骨髓间充质干细胞向软骨细胞分化的比较
A comparative study on bone marrow mesenchymal stem cells differentiated into chondrocytes induced by co-culture with chondrocytes and conditioned medium
摘要
Abstract
BACKGROUND: In vitro differentiation of bone marrow mesenchymal stem cells (BMSCs) is dependent on appropriate culture conditions to a great extent.OBJECTIVE: To study the biological characteristics of chondrocytes differentiated from BMSCs co-cultu red with chondrocytes versus cultured in conditioned medium.METHODS: Rat BMSCs and chondrocytes were isolated and cultured in neural induction. Cellular non-contact co-culture induced method and conditioned medium induced method were taken to reduce BMSCs differentiating into chondrocytes. And the biological characteristics of the cells were evaluated by MTT, mitotic cycle, GAG secretion, RT-PCR, cell-staining methods,immunocytochemistry.RESULTS AND CONCLUSION: The biological characters of co-culture induced cells were seemed to be better than those of conditioned medium induced cells, which point out that BMSCs co-cultured with chondrocytes are appropriate as seed cells in cartilage tissue engineering.关键词
共培养/软骨细胞/骨髓间充质干细胞/诱导分化/组织工程分类
医药卫生引用本文复制引用
吕晶,柯杰,王琳,张冬梅,刘冰,逄键梁,邓天政..与软骨细胞共培养和条件培养液诱导骨髓间充质干细胞向软骨细胞分化的比较[J].中国组织工程研究与临床康复,2011,15(19):3421-3426,6.基金项目
国家自然科学基金,青年科学基金(30900314)"组织工程化骨-软骨组织构建过程中血管化与血管抑制的研究" （30900314）
国家自然科学基金,青年科学基金(81000423)"下齿槽神经缺失对下颌骨牵张成骨影响的蛋白质组学分析". （81000423）
