中国组织工程研究与临床康复2011，Vol.15Issue(19)：3427-3432,6.DOI:10.3969/j.issn.1673-8225.2011.19.002
体外诱导胎儿骨髓间充质干细胞向胰岛β样分泌细胞的分化
In vitro differentiation of fetal bone marrow mesenchymal stem cells into islet beta like cells
郭嘉宁 1雷建园 1李奕 1畅继武1
作者信息
- 1. 天津市泌尿外科研究所肿瘤免疫室,天津市,300211
- 折叠
摘要
Abstract
BACKGROUND: There is no a mature program for identifying islet like cells differentiated from human bone marrow mesenchymal stem cells (BMSCs). OBJECTIVE: To investigate the possibility of human fetal BMSCs differentiating into insulin-secreting cells in appropriate condition. METHODS: The fetal BMSCs were co-cultured with fetal insulin cells on Transwell dou ble-layer culture plate. In the control group, the BMSCs were cultured in culture medium containing islet cells. Morphological changes in fetal BMSCs and islet cells were analyzed under a phase contrast microscopy. The morphological changes and insulin secretions of BMSCs under the stimulation of fetal islet cells were detected by radioimmunoassay. RESULTS AND CONCLUSION : The fetal BMSCs were isolated and purified, identical cell morphology liked plants spindle-like arrangement. Fetal islet cells were confirmed by panccreatic β endocrine cells under electron microscopy. The undifferentiated BMSCs exhibited were adherent and long spindle-shaped cells. After induction, cells gradually became round and formed clusters. Cells secreted abundant insulin and formed islet-like cell clusters that exhibited positive dithizone staining and immunocytochemical staining at 9 days. No insulin was detected in the control group. It is indicated that fetal BMSCs have the potentiality to differentiate into insulin-producing cells in appropriate condition .关键词
间充质干细胞/胰岛细胞/共培养/免疫细胞化学/双硫腙染色分类
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郭嘉宁,雷建园,李奕,畅继武..体外诱导胎儿骨髓间充质干细胞向胰岛β样分泌细胞的分化[J].中国组织工程研究与临床康复,2011,15(19):3427-3432,6.
