中国组织工程研究与临床康复2011，Vol.15Issue(19)：3443-3446,4.DOI:10.3969/j.issn.1673-8225.2011.19.005
骨髓源干细胞参与的血管内皮更新
Bone marrow-derived stem cells involved in the renewal of vascular endothelium
摘要
Abstract
BACKGROUND: Bone marrow-derived stem cells have strong plasticity, but the mechanism that they participate in regeneration and renewal of tissue is still conflicting between transdifferentiation and cell fusion.OBJECTIVE: To investigate the possibility and possible mechanism of bone marrow-derived stem cells involvement in the renewal of vascular endothelial cells in different organs through sex crossing bone marrow transplantation using mouse models of sexual mosaic.METHODS: Female C57BL/6-green fluorescent protein (GFP) mice were used as donor mice, and male C57BL/6 mice were used as the recipient to establish bone marrow transplanted chimeric mice. The chimerism of bone marrow was assessed by flow cytometry. Twenty weeks after transplantation, frozen slide of the brain, kidney, liver, spleen, and heart were prepared and fluorescence in situ hybridization was applied to label the Y chromosome, then GFP expression and Y chromosome were observed using fluorescence microscope.RESULTS AND CONCLUSION: The percent of GFP+ cell of bone marrow cells in the recipient was (7.48±1.38)％ and (73.92±5.57)％ at week 1 and week 4 respectively, indicating the success of sex crossing bone marrow transplantation model. In week 20, GFP expression was detected in vascular endothelial cells in the brain, kidney, liver, and spleen, and some cells also showed Y chromosome expression, indicating cell fusion occurred. No GFP expression was observed within the parenchyma of the brain and heart. Results have indicated that bone marrow-derived stem cells might have participated in the renewal of vascular endothelial cells in different organs through cell fusion.关键词
骨髓移植/干细胞/转基因小鼠/内皮细胞/细胞融合分类
医药卫生引用本文复制引用
许翼麟,何向辉,魏伟,章志翔,朱理玮..骨髓源干细胞参与的血管内皮更新[J].中国组织工程研究与临床康复,2011,15(19):3443-3446,4.基金项目
国家自然科学基金资助项目(30870967),课题名称"骨髓源干细胞融合促进恶性肿瘤发生和进展的实验研究". （30870967）
