安徽农业科学2011，Vol.39Issue(10)：6075-6076,2.
高效液相色谱法快速测定植物油中苯并芘的研究
Fast Analysis of Benzopyrene in Vegetable Oil by HPLC
吴海智 1周丛 1袁列江 1黄亮 1肖克 1吉彬彬 1梁军1
作者信息
- 1. 湖南省产商品质量监督检验院,湖南长沙,410007
- 折叠
摘要
Abstract
[ Objective ] The aim was to develop a HPLC method for the determination of benzopyrene in vegetable oil. [ Method ] With column of C18(5 μ,m,4.6 mm ×250 mm) ,the mobile phase of acetonitrile:water (88: 12) ,the flow of 1.0 ml/min,excitation wavelength of 384 nm,emission wavelength of 406 nm,column temperature of 35 ℃. and the injection volume of 10 μl ,the residual of benzopyrene in vegetable oil was determined. [ Result]The limit of detection was 1 μg/kg,The linear plots were obtained between 0 and 0. 1 μg/ml. Overall recoveries were more than 95％ ,and RSD was 1.04％. [ Conclusion]This method has advantages of simple process of sample treatment, short analysis time and low consumption of reagents,and is suitable for the quantitative and qualitative determination of benzopyrene in vegetable oil.关键词
苯并芘/高效液相色谱法/植物油Key words
Benzopyrene/ High performance liquid chromatography/ Vegetable oil分类
农业科技引用本文复制引用
吴海智,周丛,袁列江,黄亮,肖克,吉彬彬,梁军..高效液相色谱法快速测定植物油中苯并芘的研究[J].安徽农业科学,2011,39(10):6075-6076,2.
