安徽农业科学2011，Vol.39Issue(8)：4710-4713,4.
抑制消减杂交技术筛选鹅产蛋期差异表达基因
Differentially Expressed Geese Genes in Egg Laying Period by Using SSH
摘要
Abstract
[ Objective ] The aim was to identify and screen differential expression genes between egg - laying and broodiness phase. [ Method] SSH was applied to construct directional cDNA libraries of geese egg-laying and broodiness ovarian tissue, thus, differential expression genes were obtained. [ Result ] Positive clones from libraries were randomly selected to validate with PCR, the results showed that the quality of libraries were perfect. Consequently, 654 positive clones were selected to sequence, qualify, cluster, assemble and comparing through Blast.166 differentially expressed gene fragments from two libraries showed homology to other known sequences, including 92 functional genes.[ Conclusion] Comparing broodiness library with SSH library, it indicated that some laying-specified genes involved in metabolism and cell defense; on the other hand, some broodiness-specified genes involved in apoptosis, cell organization, signal transduction. The results provided the basis for future revealing the mechanism on ovarian follicles development and molecular marker of fertility.关键词
鹅/繁殖性能/卵巢/抑制消减杂交技术/表达序列标签Key words
Geese/ Reproductive performance/ Ovary/ SSH/ ESTs分类
农业科技引用本文复制引用
郭军,章双杰,邹剑敏,陆火林,苏建东,汤青萍..抑制消减杂交技术筛选鹅产蛋期差异表达基因[J].安徽农业科学,2011,39(8):4710-4713,4.基金项目
国家科技支撑计划项目(2008BADB2B08) （2008BADB2B08）
江苏支撑计划(BE2010370) （BE2010370）
扬州农业攻关项目(Y722009034). （Y722009034）
