中国组织工程研究与临床康复2011，Vol.15Issue(25)：4579-4582,4.DOI:10.3969/j.issn.1673-8225.2011.25.006
NaOH消蚀法制备脱细胞真皮基质修补腹壁疝
Acellular dermal matrix prepared by NaOH-maceration for ventral hernia repair
摘要
Abstract
BACKGROUND: The use of an acellular dermal matrix (ADM) may represent a new alternative to prosthetic mesh products.OBJECTIVE: To study the value of porcine ADM made by NaOH-maceration as ventral hernia-repairing material.METHODS: ADM was made from the full thick skin of the back of swines by NaOH-maceration. Forty-five male SD rats were randomly divided into three groups including ventral hernia group (n=7), ADM group (the ventral hernia repaired by 3.5 cm× 4.0 cm ADM, n=28) and Marlex mesh group (the ventral hernia repaired by 3.5 cm×4.0 cm Marlex mesh, n=10). The occurrence of ventral hernia was observed postoperatively 1 week. To evaluate ADM histology, 4 rats in ADM group were sacrificed at 1, 2, 3,5 and 10 weeks after surgery respectively; 6 rats were respectively selected from the Marlex group and ADM group for the experiment of tension resistance at 5 weeks after surgery.RESULTS AND CONCLUSION: The occurrence of ventral hernia in ADM group and Marlex mesh group was significantly lower than that of ventral hernia group 1 week after surgery (P ＜ 0.001), but there was no difference between ADM group and Marlex mesh group(P ＞ 0.05). The collagen of ADM did not change much after implantation. ADMs became infiltrated with little fibroblasts by 1 week after surgery. New wessels in ADMs were found by 2 weeks after surgery. The vessel density of ADMs kept stable by 5 weeks after surgery. The Marlex had a significantly higher breaking strength than that of the ADM (P ＜ 0.001). But 5 weeks after surgery, the tension resistance of ADM-fascial interface was higher than that of the Marlex-fascial interface (P ＜ 0.05). ADM prepared by the maceration of NaOH can be used as the material repairing ventral hernia.关键词
脱细胞真皮基质/脱细胞真皮基质-筋膜组织/疝/修补材料/NaOH消蚀法/生物材料分类
医药卫生引用本文复制引用
王军,张宝良,袁庆鑫,米立国..NaOH消蚀法制备脱细胞真皮基质修补腹壁疝[J].中国组织工程研究与临床康复,2011,15(25):4579-4582,4.基金项目
河北省卫生厅科研基金(04106). （04106）
