中国组织工程研究与临床康复2011，Vol.15Issue(25)：4583-4586,4.DOI:10.3969/j.issn.1673-8225.2011.25.007
缓释型重组人骨形态发生蛋白2/壳聚糖生物骨修复材料诱导骨形成
Enhancement of bone formation by recombinant human bone morphogenetic protein-2/chitosan bone biomaterials
摘要
Abstract
BACKGROUND: The recombinant human bone morphogenetic protein-2 (rhBMP-2) administered in solution often loses its bioactivity in a short time and does not always exhibit efficacy required in bone regeneration in vivo. It would be ideal to develop a system for the sustained delivery of biologically active rhBMP-2 over an extended period of time.OBJECTIVE: To evaluate rhBMP-2 sustained delivery and osteoinductive effects of bone biomaterials which were combined with chitosan films containing rhBMP-2.METHODS: ①The rhBMP-2 was added into a chitosan solution to prepare chitosan films which were spread on the bone materials. The effect of slow release was analyzed by ELISA assays. ②In vitro, the osteoinductive to differentiation marker (osteocalcin) of C2C12 cells after induction of rhBMP-2/chitosan (CS) bone biomaterials was assayed by Alizarin red staining.③Intramuscular implantation test was made in vivo, rhBMP-2/CS carriers were implanted in muscles of the hind leg of rat for two weeks. The amount of calcium deposited in the implants was measured to assay bioactivity.RESULTS AND CONCLUSION: ①A scanning electron micrograph of the rhBMP-2/CS bone biomaterials showed uniform distribution of chitosan films on the material surface with rhBMP-2 scattering like cluster. ②An abrupt rhBMP-2 release from rhBMP-2/CS carriers was observed. 50％ of the loaded rhBMP-2 was released in 4 days. Thereafter, the release rate was nearly constant until day 12 when 90％ of rhBMP-2 was released. All of it was released by day 18 at last. ③In vitro rhBMP-2 released from the rhBMP-2/CS carriers stimulated an increase in osteocalcin of C2C12 cells after 4 weeks. ④In vivo bone formation studies showed the rhBMP-2/CS biomaterials induced bone formation to a much greater extent than control groups by Ca2+ test assay. These results demonstrate that the rhBMP-2/CS bone biomaterials delivery system is capable of potentiating the osteogenic efficacy of rhBMP-2 and underscore its importance as a possible bone regeneration strategy.关键词
人骨形态发生蛋白2/壳聚糖/缓释/骨修复材料/骨形成分类
基础医学引用本文复制引用
王丁丁,汪炬,曾戎,杨敏儿,何鉴贤,陈阳,何水连,陈安安,周智优,袁丽颖..缓释型重组人骨形态发生蛋白2/壳聚糖生物骨修复材料诱导骨形成[J].中国组织工程研究与临床康复,2011,15(25):4583-4586,4.基金项目
广东省科技攻关项目(2009B030801237) （2009B030801237）
广东省教育部科技部企业科技特派员行动计划项目(2009B090600134) （2009B090600134）
国家自然科学基金青年基金项目(81000923). （81000923）
