中国组织工程研究与临床康复2011，Vol.15Issue(25)：4591-4596,6.DOI:10.3969/j.issn.1673-8225.2011.25.009
无细胞神经支架复合骨髓间充质干细胞构建组织工程人工神经修复坐骨神经缺损
Acellular nerve scaffold loading bone marrow mesenchymal stem cells to prepare artificial nerve for sciatic nerve defects
摘要
Abstract
BACKGROUND: The authors have successfully prepared an acellular nerve graft to be combined with bone marrow mesenchymal stem cells in artificial nerve bridge of rat sciatic nerve defects.OBJECTIVE: To investigate the motor function of rats with sciatic nerve defects following repairing using acellular nerve graft combined with bone marrow mesenchymal stem cells.METHODS: Adult SD rats were used to prepare rat models of 15-mm sciatic nerve defects. Tissue-engineered nerve, tissue-engineered nerve scaffold, and autogenous nerve were separately applied to bridge sciatic nerve defects. After 20 weeks, electrophysiological determination of regenerated nerves was conducted, wet weight of the anterior tibial muscle was determined,and histological and transmission electron microscopy analyses of the gastrocnemius was done.RESULTS AND CONCLUSION: Twenty weeks after nerve grafting, no difference in the wet weight of the anterior tibial muscle was shown to be significant between tissue-engineered nerve and autogenous nerve grafts (P ＞ 0.05). The nerve conduction velocity was (30.56±2.15) m/s. The findings indicate that the acellular nerve graft combined with bone marrow mesenchymal stem cells can promote the recovery of motor function in rats with sciatic nerve defects.关键词
组织工程人工神经/移植/腓肠肌/坐骨神经缺损/功能恢复分类
基础医学引用本文复制引用
张彩顺,吕刚..无细胞神经支架复合骨髓间充质干细胞构建组织工程人工神经修复坐骨神经缺损[J].中国组织工程研究与临床康复,2011,15(25):4591-4596,6.基金项目
辽宁省教育厅高校创新团队项目 (2008T114),课题名称:组织工程人工神经桥接大鼠坐骨神经缺损的实验研究. （2008T114）
