中国组织工程研究与临床康复2011，Vol.15Issue(25)：4615-4618,4.DOI:10.3969/j.issn.1673-8225.2011.25.014
复合壳聚糖纳米微球聚乳酸-羟基乙酸/纳米羟基磷灰石缓释载体系统对蛋白的缓释作用
Delayed release of compound chitosan nano-microspheres PLGA/nHA control-releasing carrier systems
安世昌 1孙健 1李亚莉 1许尧详 1王科 1陈立强 1肖文林1
作者信息
- 1. 青岛大学医学院附属医院口腔颌面外科,山东省青岛市,266021
- 折叠
摘要
Abstract
BACKGROUND: Polylactic acid-glycolic acid (PLGA) is a kind of biodegradable polymer with excellent biocompatibility, plasticity and certain strength and toughness. But its degradation products are acidic, which can affect local pH and inhibit tissue growth. OBJECTIVE: To prepare composite scaffolds with excellent characteristics of delayed release of proteins.METHODS: Using bovine serum album (BSA) as model protein, chitosan microspheres (CMs) were prepared by ionotropic gelation. Ice particulates were used as porogen. Composite scaffolds were prepared with CMs/nHA/PLGA by freeze-drying.Scanning electron microscope, transmission electron microscope, mercury porosimeter and universal testing machine ware used to observe the characteristic and morphology of the composite scaffolds.RESULTS AND CONCLUSION: The CMs were spherical in shape with a regular surface. The diameters of the CMs were in the range of 220-770 nm, mainly ranging 380-650 nm. The encapsulation efficiency of the CMs was 68.3％, and the loading capacity was 39.2％. The both were related with the initial concentration of BSA. The pore diameter of the composite scaffolds was about 125-355 m,the porosity was 83.4％, and the compressive strength was about 1.4-2.1 MPa. The cumulative degradation was 28.6％ at 10 weeks. The cumulative release of BSA from PLGA/nHA scaffolds was 85％ in 2 days, which from CMs and CMs/nHA/PLGA scaffolds was 48.9％ and 35.7％ in 9 days. The CMs microspheres and CMs/nHA/PLGA scaffolds have a desirable release rates for BSA and strength, are expected to use as carrier for growth factor and bone tissue engineering scaffolds.关键词
复合支架/壳聚糖/聚乳酸-羟基乙酸/缓释载体/骨组织工程分类
医药卫生引用本文复制引用
安世昌,孙健,李亚莉,许尧详,王科,陈立强,肖文林..复合壳聚糖纳米微球聚乳酸-羟基乙酸/纳米羟基磷灰石缓释载体系统对蛋白的缓释作用[J].中国组织工程研究与临床康复,2011,15(25):4615-4618,4.
