中医药信息2011，Vol.28Issue(2)：19-21,3.
黄芪含药血清对H2O2诱导人脐静脉内皮细胞氧化损伤模型细胞活性的影响
The Effect of Astragalus Medicated Serum on Cell Viability of Oxidative Injured Model of H2O2 induced HUVEC
摘要
Abstract
Objective :Yang Xin Tang Fong single herb Astragalus serum contains different concentrations of H2O2 induced human umbilical vein endothelial cells (HUVEC) oxidative damage model of cell proliferation and the protective effect of HUVEC on the injury to further research to support Coronary heart disease with unstable angina exact mechanism of action. Methods: Astragalus liquid concentration 1.03g· mL-1 fed rats 1 week after the last administration blood centrifugation preparation containing serum. The concentration of HUVEC with different doses (2％ ,4％ ,6％ ) single Chinese Astragalus containing serum, basal medium, control serum with culture medium, divided into 5 groups, the role of culture 24h after adding H2O2 2h, by MTT method and morphological effects of different concentrations of Astragalus containing serum on the activity of H2O2 injury in HUVEC growth. Results:Astragalus containing serum 2％ ,4％ ,6％ OD values in each group which was compared with model control group was significantly higher H2O2, and H2O2 model group was statistieally significant compared ( P ＜ 0. 01 ), and 2％, 4％, 6％ group was significantly higher than ( P ＜0. 05 ). Morphological, Astragalus containing serum induced an smaller, space becomes larger, but there is a link between each other, less exfoliated cells; significant improvement in the degree of shrinkage. Conclusion:Astragalus containing serum can promote H2O2 injury in human umbilical vein endothelial cell proliferation,enhance the ability of HUVEC against oxidative damage, reversal, improve endothelial dysfunction, and has a dose - dependent.关键词
黄芪/不稳定型心绞痛/内皮细胞增殖率/MTT分类
医药卫生引用本文复制引用
张继江,刘影哲,周亚滨..黄芪含药血清对H2O2诱导人脐静脉内皮细胞氧化损伤模型细胞活性的影响[J].中医药信息,2011,28(2):19-21,3.基金项目
黑龙江省自然科学基金重点项目(zjy0606-02) （zjy0606-02）
