肿瘤防治研究2011，Vol.38Issue(3)：250-253,4.DOI:10.3971/j.issn.1000-8578.2011.03.003
Ca2+在鼻咽癌细胞凋亡性容积减小中的作用
Roles of Ca2+ in Apoptotic Volume Decrease in Nasopharyngeal Carcinoma Cells
摘要
Abstract
Objective To study the role of calcium signaling pathway in apoptotic volume decrease (AVD) in nasopharyngeal carcinoma cells. Methods The apoptotic inducer Cisplatin was used to induce apoptotic volume decrease in poorly differentiated nasopharyngeal carcinoma cells (CNE-2Z cells). The software Q500MC was employed for acquisition of time-lapse images of living cells. Cell volume changes were obtained by analysis of the image analysis software Scion Image. Results Extracellular application of Cisplatin induced a decrease of cell volume. The Cisplatin-induced changes in cell volume were significant 10 min after exposed to bath solution containing Cisplatin. Depletion of calcium from the bath solution or extracellular applications of calcium channel blocker nifedipine delayed the initiation of AVD induced by Cisplatin, but not the appearance of AVD. There was no significant difference in AVD 50 min after applications of Cisplatin between control and cells treated with nifedipine or Ca2+ -free bath solution. Conclusion The inflow of Ca2+ across the cell membrane may play an important role in activation of AVD at the early stage of apoptosis.关键词
鼻咽癌/凋亡性容积减小/Ca2+信号分类
医药卫生引用本文复制引用
向静瑶,胡代军,孙利华,杨林杰,左婉红,朱林燕,陈丽新,王立伟..Ca2+在鼻咽癌细胞凋亡性容积减小中的作用[J].肿瘤防治研究,2011,38(3):250-253,4.基金项目
国家自然科学基金资助项目(30771106,30870567,30871267) （30771106,30870567,30871267）
暨南大学本科生科技创新工程资助项目(cx08099) （cx08099）
