中国农业科学2011，Vol.44Issue(5)：925-932,8.DOI:10.3864/j.issn.0578-1752.2011.05.008
DHN黑色素与玉米大斑病菌附着胞膨压形成的关系
Relationship Between DHN Melanin and Formation of Appressorium Turgor Pressure of Setosphaeria turcica
摘要
Abstract
[Objective] The objective of this study is to discuss the effect of intracellular DHN melanin ofSetosphaeria turcica on the appressorium turgor pressure generated and elucidate the invasive mechanism of S. turcica. [Method] In this research, the best conditions for appressorium formation of S. turcica were determined by inducing appressorium production. The appressorium cell wall pore sizes and turgor pressures of wild-type isolate 01-23 and melanin-deficient strains ΔSt3hnr of S. turcica were measured by a solute exclusion technique and incipient-cytorrhysis technique. [Result] The appressorium pore size of wild-type isolate 01-23 was 2.1-2.7 nm and it had a 5.4 MPa turgor pressure, while it was shown a range of 2.7-3.3 nm in melanin-deficient strainsΔSt3hnr and a lower pressure about 4.1 MPa. The results indicated that appressorium without melanin could not form a high turgor pressure resulting in losing the ability for penetrating. [ Conclusion] It is suggested that the melanin layer is significant in accumulation of excess turgor pressure and the turgor pressure of S. turcica play a major role in substance penetration.关键词
玉米大斑病菌/附着胞/溶质排斥技术/incipient-cytorrhysis技术/膨压引用本文复制引用
曹志艳,贾慧,朱显明,董金皋..DHN黑色素与玉米大斑病菌附着胞膨压形成的关系[J].中国农业科学,2011,44(5):925-932,8.基金项目
国家自然科学基金项目(30771390) （30771390）
