中国农业科学2011，Vol.44Issue(5)：1063-1070,8.DOI:10.3864/j.issn.0578-1752.2011.05.025
圩猪H-FABP基因多态性分析及其与IMF含量的相关性
Polymorphism Analysis of H-FABP Gene and Correlation with IMF Content in Wei Pig
摘要
Abstract
[Objective] Polymorphism of heart fatty acid-binding protein (H-FABP) gene and its correlation with IMF content in Wei pig were studied. [Method] PCR-RFLP (HinfI, HaeⅢ, Msp Ⅰ 3 kinds of restriction enzymes) and molecular marker techniques were used in the study and the genetic variation of H-FABP gene 5'-upstream region and second intron in 123 Wei pigs was analyzed. [Result] In 5'-upstream region, there was polymorphism in loci of the Hinf Ⅰ and the frequency of allele H was 0.6545, showing moderate polymorphism (PIC = 0.3500). In the second intron, polymorphism was detected in polymorphic loci Hinf Ⅰ *, the frequency of allele B was 0.7195, showing moderate polymorphism (PIC = 0.3222). Loci of HaeⅢ also existed, allele D frequency was 0.6301, showing moderate polymorphism (PIC = 0.3575 ). While no polymorphism was detected in the loci of Msp Ⅰ, which the genotype was AA genotype. The gene frequencies and genotype frequencies of pigs tested have reached the Hardy-Weinberg equilibrium (P＞0.05). The detection of the genotype of the IMF content had the trend of Hh＞Hh＞hh, dd＞Dd＞DD, BB＞Bb＞bb, genetic effect values were as following: 4.6918, 4.2665, 3.7712, 4.6124, 4.3167, 3.8173, 4.3042, 4.2358, and 4.1970. Cloning and sequencing analysis on polymorphic fragments of three PCR-RFLP in H-FABP gene showed that Hinf Ⅰ -RFLP is due to T→C mutation in 1 324 bp, HaeⅢ-RFLP is due to C→G mutation in 1 811 bp, Hinf Ⅰ *- RFLP is due to C→T mutation in 1 970 bp. Sequencing results had 97.9％ and 98.3％ homology with the sequence X98558 and Y16180 published by GenBank.[Conclusion] Polymorphisms in 5'-upstream region and the second intron of H-FABP gene had certain effect on IMF content in Wei pig. However, whether these polymorphisms are important genetic markers, which are related with H-FABP gene and IMF content in Wei pig, needs to be further studied.关键词
圩猪/H-FABP基因/IMF含量/序列分析/PCR-RFLP引用本文复制引用
张陈华,王阳,丁月云,殷宗俊..圩猪H-FABP基因多态性分析及其与IMF含量的相关性[J].中国农业科学,2011,44(5):1063-1070,8.基金项目
教育部科学技术研究重点项目(208059)、安徽省"十一五"科技攻关项目(07010302136)、安徽省自然科学基金重点项目(KJ2009A114) （208059）
