医药导报2011，Vol.30Issue(6)：787-788,2.DOI:10.3870/yydb.2011.06.033
反相高效液相色谱法测定地塞米松软膏中地塞米松含量
Content Determination of Dexamethasone in Dexamethasone Ointment by RP-HPLC
杨习江 1哈娜 1哈力1
作者信息
- 1. 哈尔滨医科大学附属第一临床医院药学部,150001
- 折叠
摘要
Abstract
Objective To develop a method for content determination of dexamethasone in dexamethasone ointment by RP-HPLC. Methods The separation was performed on Sinochrom ODS-BP( 4.6 mm×250 mm ,5 μm )column with methanol:water( 80∶ 20 ) as a mobile phase, and with a wavelength of UV detector at 240 nm. The flowing rate was 1.0 mL · min -1 and the loading volumn was 20 μL. Results The linear range of dexamethasone was within 0.25 -2.50 μg ( r = 0. 999 9 ). The average recovery of dexamethasone was 100. 1％ with RSD as 0.75％ ( n = 9 ). Conclusion The method is simple, rapid,accurate and reliable, which can be applied to the quality control of dexamethasone ointment.关键词
地塞米松/色谱法,高效液相,反相/含量测定分类
医药卫生引用本文复制引用
杨习江,哈娜,哈力..反相高效液相色谱法测定地塞米松软膏中地塞米松含量[J].医药导报,2011,30(6):787-788,2.
