临床肝胆病杂志2011，Vol.27Issue(3)：248-250,3.
siRNA抑制IGFBPrP1表达对肝星状细胞株分泌细胞外基质的影响
Effects of knocking down IGFBPrP1 with siRNA on extracellular matrix secretion in HSC-T6
摘要
Abstract
Objective To investigate the effects of inhibiting insulin-like growth factor binding protein related protein1(IGFBPrP1)by chemically synthesized small interfering RNA(siRNA) on the secretion of extracellular matrix(ECM) in HSC-T6 and to determine the effect of IGFBPrP1 on the hepatic fibrosis.Methods (1)Two pairs of chemically synthesized siRNAs targeting IGFBPrP1 were respectively transfected into HSC-T6 cells for evaluation of silence efficacy and the one with higher efficacy was used in the following experiments; (2)Divide the HSC-T6 cells into three groups as follows: normal control group, negative control group and IGFBPrP1 siRNA group.HSC-T6 was treated by IGFBPrP1 siRNA, then the expressions of IGFBPrP1 and collagenⅠand fibronectin were detected by Western Blot.The data was analyzed by one-way ANOVA and SNK-q test.Results (1)Two pairs of siRNAs were transfected into HSCT6.One pair of siRNA, which can highly effectively inhibit expression of IGFBPrP1 gene was screened successfully; (2)The expressions of IGFBPrP1 and collagenⅠand fibronectin in IGFBPrP1 siRNA group were significantly decreased than those in normal control group and negative control group(F﹦48.018, 81.246, 43.850, P﹤0.01).Conclusion siRNA targeting IGFBPrP1 specifically inhibits the expression of IGFBPrP1 in HSC resulting in decreased synthesis and secretion of collagenⅠand fibronectin in hepatic stellate cells.So IGFBPrP1 can play an important role in liver fibrosis.关键词
RNA,小分子干扰/胰岛素样生长因子结合蛋白质类/肝细胞/细胞外基质分类
医药卫生引用本文复制引用
刘立新,邱志红,张骞骞..siRNA抑制IGFBPrP1表达对肝星状细胞株分泌细胞外基质的影响[J].临床肝胆病杂志,2011,27(3):248-250,3.基金项目
国家自然科学基金(30871146) （30871146）
教育部新世纪优秀人才支持计划(NCET-06-0264) （NCET-06-0264）
