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采用InDel和SSR标记分析番茄品种基因组DNA多态性

申璐 沈火林 柴敏 王银磊 杨文才

中国农业大学学报2011,Vol.16Issue(2):34-42,9.
中国农业大学学报2011,Vol.16Issue(2):34-42,9.

采用InDel和SSR标记分析番茄品种基因组DNA多态性

Genomic DNA polymorphisms in tomato varieties revealed by InDel and SSR markers

申璐 1沈火林 1柴敏 2王银磊 1杨文才1

作者信息

  • 1. 中国农业大学农学与生物技术学院,北京,100193
  • 2. 北京市农林科学院蔬菜研究中心,北京,100097
  • 折叠

摘要

Abstract

The emergency of genomic sequences in tomato (Solanum lycopersicurn L. ) provides a base for developing InDel markers. In this study, 18 InDel and 22 SSR markers were used to analyze genomic DNA diversity in 59 Chinese and 23 US fresh-market tomato varieties to investigate the potential use of InDel markers in tomato molecular breeding.Comparison between 59 Chinese and 23 US varieties were also conducted at marker level. The results indicated that InDel marker showed lower polymorphisms than SSR markers in 82 varieties. The number of alleles amplified by InDel primers was significantly lower than that amplified by SSR markers in Chinese varieties. But no obvious difference was observed in US varieties. The number of alleles amplified by InDel primers was significantly lower in Chinese varieties than in US varieties. Cluster analysis indicated that genetic difference was very low among varieties developed by Chinese breeding programs but relatively high between Chinese and foreign varieties.

关键词

番茄/InDel/SSR/标记多态性/基因组DNA多样性

分类

农业科技

引用本文复制引用

申璐,沈火林,柴敏,王银磊,杨文才..采用InDel和SSR标记分析番茄品种基因组DNA多态性[J].中国农业大学学报,2011,16(2):34-42,9.

基金项目

北京市果类蔬菜产业创新团队资助 ()

新世纪优秀人才支持计划(NCET-08-0531) (NCET-08-0531)

中国农业大学学报

OA北大核心CSCDCSTPCD

1007-4333

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