中国农业大学学报2011，Vol.16Issue(2)：125-132,8.
饲料中硝基呋喃类药物高效液相色谱检测方法的建立
Establishment of HPLC method for determination of nitrofurans in feeds
摘要
Abstract
To detect nitrofurans in feeds,a high performance liquid chromatography (HPLC) method for determination of nitrofurans, such as Furacillin, Nitrofurantion, Furazolidone, and Furaltadone, was established in this study. Nitrofurans in feeds was extracted by acetonitrle, concentrated extraction was cleaned up with HLB solid phase extraction (SPE) column. Nitrofurans were separated on Water Sunfire C18 column with UV detector 365 nm, using acetonitrile and ammonium acetate buffer as mobile phase. The results showed that the nitrofurans standard curve regression coefficient is above 0. 9997, and the linearity range is 0.2～30.0 μg/mL. The average recoveries of nitrofurans ranged from 70％～98％ ,with RSD below 10％. The detection limit of Furacillin, Nitrofurantion, Furazolidone and Furaltadone was 0. 020,0.020,0. 020 and 0. 100 μg/mL, respectively ; and the quantitation limit was 0.50,0.50,0.50 and 1.00 mg/kg,respectively. This method exhibits uncomplicated operation, excellent linearity, high sensitivity and superior reproducibility for determination of nitrofurans residues in formula feed,concent-rate feed and premixes feed.关键词
饲料/高效液相色谱法/固相萃取/呋喃类药物分类
农业科技引用本文复制引用
王蕾,鲍恩东..饲料中硝基呋喃类药物高效液相色谱检测方法的建立[J].中国农业大学学报,2011,16(2):125-132,8.基金项目
农业部农业行业标准制定项目(农财发[2009]105号-50) （农财发[2009]105号-50）
