中国水产科学2011，Vol.18Issue(2)：245-255,11.DOI:10.3724/SP.J.1118.2011.00245
中国近海习见头足类DNA条形码及其分子系统进化
DNA Barcoding for Cephalopoda in classification and phylogeny
摘要
Abstract
The mitochondrial cytochrome c oxidase subunit I genes (CO I) were sequenced from 97 individuals out of 11 common species of Cephalopoda in China by using the universal barcoding primers. The 97 sequences were compared with the other 95 homologous sequences belonging to 19 species of Cephalopoda obtained from the GenBank. As a result, 23.33％ of the Cephalopoda species' COI sequences included Insert-Deletion sites. The indels of COI sequences in Uroteuthis duvauceli counted up to 33. The COI sequences of Cephalopoda present the base preference. On average, the content of A+T (66.70％) was significantly higher than that of G+C (33.30％). As calculated by Kimera-2-parameter model, the mean distance pairwise-species and within-species was 0.202 4 and 0.007 2, respectively. The valid name and taxonomy of swordtip squid (Loligo edulis or Uroteuthis edulis or Photololigo edulis) remain controversial or unresolved. Here data gathered using COI genes does not support the assertion that swordtip squid should position in the genera of Loligo and Uroteuthis. In view of the fact that small genetic distance (0.012 0-0.038 5) among 6 species of Pareledone is found, suggesting that the threshold of barcodes (0.02) to identify species be worth furthering studies, particularly for those species with low genetic variation. According to maximum parsimony and neighbor joining trees for all 192 sequences of Cephalopoda, the consistency with morphological classification on both species and genus levels was much higher (100％, 91.67％) than that of family or order levels (80％, 66.67％). Consequently, providing essential revision and supplement to morphology taxonomy, the COI barcoding is a useful tool for both identification and phylogeny of Cephalopoda.关键词
头足类/COI基因/DNA条形码/分类鉴定/系统进化分类
生物科学引用本文复制引用
王鹤,林琳,柳淑芳,姜志强,庄志猛..中国近海习见头足类DNA条形码及其分子系统进化[J].中国水产科学,2011,18(2):245-255,11.基金项目
国家自然科学基金项目(40776097,31061160187) （40776097,31061160187）
公益性行业(农业)科研专项(200903005). （农业）
