中国水产科学2011，Vol.18Issue(2)：350-359,10.DOI:10.3724/SP.J.1118.2011.00350
金乌贼和曼氏无针乌贼胚胎发育及其盐度耐受能力的比较研究
A comparative study of Sepia esculenta and Sepiella maindroni on embryonic development and ability of salinity tolerance
摘要
Abstract
Fertilized eggs of Sepia esculenta and Sepiella maindroni were collected from the wild and the embryonic development of S.esculenta was observed in laboratory.The morphological characters of fertilized eggs and newly hatched larvae between the two species were also compared.The effects of different seawater salinities of 20, 25, 30 (control), 33, 36 for S.esculenta and 15, 20, 25, 30 (control), 36 for S.maindroni on hatching rate were studied, respectively.Changes in egg fluid osmolality and egg Na+/K+-ATPase activity of S.esculenta and S.maindroni were also measured during the abrupt salinity changes of 192 h, respectively.Hatching rates of fertilized eggs at the same salinity and physiological adaptation to the abrupt salinity changes during 192 h were compared between S.esculenta and S.maindroni.The results showed that organ differentiation happened when embryo was 7-day-old, while organ formation occurred when embryo was 14-day-old for S.esculenta.The incubation period of S.esculenta fertilized egg ranged from 21 d to 22 d at 22-24℃ and salinity of 30.Hatching rates of S.esculenta and S.maindroni under control treatment were significantly higher than those under other salinity treatments (P＜0.01), indicating that salinity of 30 was the suitable hatching salinity for fertilized eggs of the two species.Fluid osmotic pressure of S.esculenta and S.maindroni fertilized egg changed as seawater salinity altered during the abrupt salinity change.The egg fluid osmotic pressure of S.esculenta was equal to the medium osmotic pressure, while the egg fluid osmotic pressure of S.maindroni was 60 mOsm/kg higher than those in the medium.Activities of Na+/K+-ATPase were not found in fertilized eggs of two species.These results indicate that fertilized eggs of two species have little ability in regulating osmotic pressure.However, fertilized egg of S.maindroni can accommodate salinity alteration better than S.esculenta.关键词
金乌贼/曼氏无针乌贼/盐度/胚胎发育/孵化率/卵液渗透压/Na+/K+-ATPase分类
农业科技引用本文复制引用
雷舒涵,吴常文,高天翔,郝振林,张秀梅..金乌贼和曼氏无针乌贼胚胎发育及其盐度耐受能力的比较研究[J].中国水产科学,2011,18(2):350-359,10.基金项目
国家863计划项目(2010AA10A404) （2010AA10A404）
国家海洋局海洋公益项目(200905019). （200905019）
