中国水产科学2011，Vol.18Issue(2)：371-378,8.DOI:10.3724/SP.J.1118.2011.00371
致病性嗜水气单胞菌生物膜的形成特性
Characteristics of biofilm formation of pathogenic Aeromonas hydrophila
摘要
Abstract
The characteristics of biofilm formation of pathogenic Aeromanas hydrophila B11 was investigated by the method of modified microtiter-plate test. The results showed that A. hydrophila B 11 developed stable and evident biofilm on the surface of the polystyrene microtiter plate, and the OD590 value of biofilm reached the secondary peak in 6 h and the highest peak in 22 h and then reduced with incubation time. The OD590 value of the biofilm decreased with densities of initial bacterial suspension. The OD590 value of biofilm recorded at 25 ℃ was significantly higher than those recorded at other temperatures(P＜0.05).A.hydrophila B11 developed evident biofilm under initial pH 5-10, while no evident biofilm was found under initial pH 3-4. When 1mmol/L magnesium ion was added, in the biofilm formation orA. hydrophila was significantly inhibited (P＜0.05), while calcium ion significantly promoted the formation of biofilm(P＜0.05). Compared with the control group, only biofilm developed on the surface of the microtiter plate coated by the extract of eel' muscle exhibited remarkably higher value of OD590(P＜0.05), while the biofilms on the microtiter plate coated by other extracts of tissues showed no remarkably difference(P＞0.05). The results indicate that: A. hydrophila B 11 strain can form biofilm on the surface of polystyrene microtiter plate; the bacterial biofilm formation can be affected by different environmental factors such as temperature, bacterial concentration, pH, Mg2+ and Ca2+coneentrations and so on; muscle extract of eel can promote the biofilm formation.[Journal of Fishery Sciences of China, 2011, 18(2): 371-378]关键词
嗜水气单胞菌/生物膜/形成特性分类
农业科技引用本文复制引用
毛秀秀,覃映雪,邹文政,郭松林,苏永全,鄢庆枇,纪荣兴..致病性嗜水气单胞菌生物膜的形成特性[J].中国水产科学,2011,18(2):371-378,8.基金项目
国家863计划资助项目(2007AA09Z115) （2007AA09Z115）
农业部行业专项(nyhyzx07-043-01) （nyhyzx07-043-01）
农业部产业技术体系项目(200907100001) （200907100001）
厦门市科技项目(3502Z20073019,3502Z20073020). （3502Z20073019,3502Z20073020）
