分析测试学报2011，Vol.30Issue(3)：302-306,5.DOI:10.3969/j.issn.1004-4957.2011.03.014
离子液体作高效液相色谱流动相添加剂测定水杨酸
Determination of Salicylic Acid by High Performance Liquid Chromatography with Ionic Liquids as Mobile Phase Additives
摘要
Abstract
A reverse phase high performance liquid chromatographic method was established for the determination of salicylic acid by using ionic liquids as mobile phase additives. The chromatographic separation of salicylic acid was performed on a ZORBAX ODS column ( 250 mm × 4. 6 mm i. d. ,5 μm). The detection of salicylic acid was carried out by ultraviolet(UV) detection. The effects of detection wavelength, pH value, concentration and alkyl chain length of ionic liquids on the separation and determination of salicylic acid were investigated. The optimum chromatographic conditions were as follows: mobile phase: methanol - 3.0 mmol · L- 1 l-butyl-3-methylimidazolium tetrafluoroborate aqueous solution( pH 3.0, adjusted with acetic acid) (60: 40, by volume), UV detection wavelength: 300 nm, flow rate: 1.0 mL · min-1, column temperature: 30 ℃. Under the optimal conditions, the retention time of salicylic acid was about 4. 4 min. The calibration curve showed a good linearity over the range of 2 - 100 mg · L-1 for salicylic acid with a correlation coefficient of 0. 999 9. The detection limit was 0. 059 mg/L. The average spiked recovery was 100％ with relative standard deviation of 0. 11％. The method was successfully applied in the determination of the content of salicylic acid in the pharmaceutical of Zujunqing Tinctura.关键词
离子液体/高效液相色谱法/水杨酸/紫外检测/药品Key words
ionic liquid/ high performance liquid chromatography/ salicylic acid/ ultraviolet detection/ pharmaceutical分类
化学化工引用本文复制引用
董影杰,于泓,黄旭,王婧菲..离子液体作高效液相色谱流动相添加剂测定水杨酸[J].分析测试学报,2011,30(3):302-306,5.基金项目
黑龙江省自然科学基金资助项目(B200909) （B200909）
