中国神经再生研究（英文版）2011，Vol.6Issue(5)：366-372,7.DOI:10.3969/j.issn.1673-5374.2011.05.008
Brain stem global gene expression profiles in human spina bifida embryos
Brain stem global gene expression profiles in human spina bifida embryos
摘要
Abstract
Environmental and genetic factors influence the occurrence of neural tube defects, such as spina bifida.Specific disease expression patterns will help to elucidate the pathogenesis of disease.However, results obtained from animal models, which often exhibit organism specificity, do not fully explain the mechanisms of human spina bifida onset.In the present study, three embryos with a gestational age of approximately 17 weeks and a confirmed diagnosis of spina bifida, as well as 3 age-matched normal embryos, were obtained from abortions.Fetal brain stem tissues were dissected for RNA isolation, and microarray analyses were conducted to examine profiles of gene expression in brain stems of spina bifida and normal embryos using Affymetrix HG-U1 33A 2.0 GeneChip arrays.Of the 14 500 gene transcripts examined, a total of 182 genes exhibited at least 2.5-fold change in expression, including 140 upregulated and 42 downregulated genes.These genes were placed into 19 main functional categories according to the Gene Ontology Consortium database for biological functions.Of the 182 altered genes, approximately 50% were involved in cellular apoptosis, growth, adhesion, cell cycle, stress, DNA replication and repair, signal transduction, nervous system development, oxidoreduction, immune responses, and regulation of gene transcription.Gene expression in multiple biological pathways was altered in the brain stem of human spina bifida embryos.关键词
apoptosis/GeneChip/gene expression/neural tube defect/spina bifidaKey words
apoptosis/GeneChip/gene expression/neural tube defect/spina bifida引用本文复制引用
Hong Zhao,Xiang Li,Wan-I Lie,Quanren He,Ting Zhang,Xiaoying Zheng,Ran Zhou,Jun Xie..Brain stem global gene expression profiles in human spina bifida embryos[J].中国神经再生研究（英文版）,2011,6(5):366-372,7.基金项目
the National Key Project of Scientific and Technical Supporting Programs funded by the Ministry of Science & Technology of China,No.2007BA107A02 （）
the National Basic Research Program of China (973Program),No.2007CB511902 （973Program）
the Shanxi Scholarship Council of China,No.2008-48 （）
the Shanxi Natural Science Foundation,No.2010011049-2 （）
the National Natural Science Foundation of China,No.31040056 （）
