中药材2011，Vol.34Issue(2)：183-186,4.
川贝母细胞团块悬浮培养生产生物碱的研究
Study on the Production of Alkaloid by Cell Mass Suspension Culture of Fritillaria cirrhosa
摘要
Abstract
Objective:Set up Fritillaria cirrhosa cell mass suspension culture system to rapidly screen the best culture conditions for cell mass proliferation and hormone combination. Methods: Using MS medium as the basic medium, the impact of inoculum size, hormone combination,growth regulators for Fritillaria cirrhosa cell mass suspension culture were compared,and also the growth of cell mass at different culture conditions was compared,and the total alkaloids content in proliferative cell mass was measured. Results and Conclusion: Fritillaria cirrhosa grow significantly faster in cell mass suspension culture than in the solid culture. The total alkaloid content in cell mass is higher than commercial and wild bulb of Fritillaria cirrhosa. The optimal inoculum size for cell mass suspension culture is 30 g/L and the optimal culture media is MS + 6-BA 2.0 mg/L + NAA 0.2 mg/L关键词
川贝母/细胞团块颗粒/悬浮培养/生物碱分类
医药卫生引用本文复制引用
王跃华,何宗晟,孙雁霞,马良良,刘益丽,林抗雪..川贝母细胞团块悬浮培养生产生物碱的研究[J].中药材,2011,34(2):183-186,4.基金项目
四川省中医药管理局(2010-79) （2010-79）
四川省科技支撑计划(2010SZ0007) （2010SZ0007）
