中药材2011，Vol.34Issue(2)：293-295,3.
鲍氏针层孔菌菌丝体多糖脱蛋白方法研究
Study on Methods of Deproteinization from Phellinus baumii Polysaccharide
摘要
Abstract
Objective:To study and compare different methods and influencing factors of deproteinization from Phellinus baumii polysaccharide. Methods:Sevag method and TCA method were used to remove protein from crude polysaccharide of Phellinus baumii,respectively. Results:The effect of TCA method on removing protein was superior to that of Sevag method,and the optimum conditions of removing protein with TCA were as follows :30min treating time,5％ trichloroacetic acid,3 times treatemt. Under these conditions the rate of protein-removed was 82％ and the rate of polysaccharide-lost was 10. 8％. Conclusion:TCA method is the optimal mean for deproteinization from Phellinus baumii polysaccharide.关键词
鲍氏针层孔菌/Sevag法/TCA法/多糖/蛋白质去除率/多糖损失率分类
医药卫生引用本文复制引用
谢丽源,张勇,彭卫红,甘炳成..鲍氏针层孔菌菌丝体多糖脱蛋白方法研究[J].中药材,2011,34(2):293-295,3.基金项目
四川省青年基金项目(2008ZQ026-072) （2008ZQ026-072）
四川省科技支撑计划项目(2008FZ0157) （2008FZ0157）
