四川中医2011，Vol.29Issue(3)：66-68,3.
RP-HPLC法测定升麻药材中升麻苷H-1含量
Determinating the Content of Cimifugoside H-1 in Cimicifugae Rhizome by RP-HPLC
摘要
Abstract
Objective: To establish an accurate method for the determination of Cimicifugoside H-lwhich was isolated from Cimicifugae Rhizome. Methods: Cimicifugoside H-1 was isolated from water-insoluble part of Cimicifuga ethanol extract by silica gel column chromatography; HPLC analysis was performed on Hypersil BDS C18 column (200mm×4.6mm, 51μm) with a mobile phase consisting of methanol: water (60: 40). The flow rate was 1.0ml/min. The detection wavelength was 210 nm and the column temperature was 30℃. Results: The linear range of Cimicifugoside H-1 was within 0.358 ～ 6. 444 mg (r=0.9996). The results that RSD of precision, stability, reproducibility were all less than 2％. The average recovery was 101.9％ and RSD was 4.81％. Conclusions: The method is simple and accurate and can be used for determinating Cimicifugoside H-1 of Cimicifugae.关键词
升麻/升麻苷H-1/RP-HPLCKey words
Cimicifugae Rhizome/Cimicifugoside H-1/RP-HPLC分类
医药卫生引用本文复制引用
靳波,刘友平,陈鸿平,赵祎姗,彭月..RP-HPLC法测定升麻药材中升麻苷H-1含量[J].四川中医,2011,29(3):66-68,3.基金项目
质检公益性行业科研专项(No.2007GYB075-2),校学生科研实践创新课题(013-011-39) （No.2007GYB075-2）
