山东医药2011，Vol.51Issue(13)：18-20,3.
CXCR7-shRNA慢病毒表达载体对人结肠癌细胞生物学特性的影响
Effects of lentiviral vector loaded CXCR7-shRNA on biological characteristics of human colon cancer cell
王红鲜 1陶霖玉 1齐柯 1向红 1张好云 1冯铎 1孔恒 1林秋生 1陈天文1
作者信息
- 1. 广东医学院附属南山医院,广东深圳,518052
- 折叠
摘要
Abstract
Objective To study the proliferative and migratory activities of human colon cancer cell after CXCR7-targeting inhibition by lentiviral vector loaded CXCR7-shRNA. Methods Human colon cancer cells HT-29 were divided into 3 groups: experimental group, negative control group and blank control group. The experimental group was administrated with LV-CXCR7shRNA, negative control group with LV-shRNA negative control and blank control group without the any.CXCR7 protein of every group were detected using Westrn blot. The proliferative and migratory activities of every group were detected using cell active assay and Transwell cell migratory assay. Results CXCR7 protein was lower expression in experimental group than the negative control group and blank control group (P ＜ 0. 05). The cell growth curve of experimental group was flatter and the level of cell growth was significantly lower than the negative control group and blank control group(P ＜0.05). The cell growth curve of negative control group was lower than that of blank control group. Transwell cell migratory assay: the cell populations of penetrating the filtrative membrane in experimental group were significantly lower than that of negative control group(P ＜0.05). Conclusions Gene silence of CXCR7-targeting suppresses the proliferative and migratory activities of colon cancer cell.关键词
结肠肿瘤/CXCR7-shRNA/细胞活性实验/细胞迁移实验分类
医药卫生引用本文复制引用
王红鲜,陶霖玉,齐柯,向红,张好云,冯铎,孔恒,林秋生,陈天文..CXCR7-shRNA慢病毒表达载体对人结肠癌细胞生物学特性的影响[J].山东医药,2011,51(13):18-20,3.
