中国药业2011，Vol.20Issue(6)：30-31,2.
复合维生素B口服液质量标准研究
Study on Quality Standard of Vitamin B Compound oral Liquid
姚忠立1
作者信息
- 1. 河南省洛阳市伊川县食品药品检验所,河南,洛阳,471000
- 折叠
摘要
Abstract
Objective To establish a TLC method for identifying hesperidin and HPLC method for simultaneously determining the contents of vitamin B1 and vitamin B6 in Vitamin B Compound Oral Liquid. Methods The Agilent 1200 HPLC instrument was utilized with acetonitrile-aqueous phase (adding heptanc sulfonic acid sodium 0.32 g, triethylamine 2 mL and glacial acetic acid 10 mLto 1 000 mL with water) (10:90) as the mobile phase, the flow velocity of 1.0 mL/min and the detection wavelength of 280 nm. Results Under this condition, good separation of the baselines between vitamin B1 and B6 could be realized and the linear ranges of vitamin B1 and B6 were 0. 262- 0.918 μg (r=0.999 9) and 0.240-0.842 μg (r=0.999 9) respectively. The average recovery rate was 98.4％ (RSD=1.70％) and 98.9％ (RSD = 1.46％ ) respectively. Conclusion This method is simple, rapid, accurate and reliable, which is suitable for the quality control of Compound Vitamin B Oral Liquid.关键词
高效液相色谱法/橙皮苷/复合维生素B/含量分类
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姚忠立..复合维生素B口服液质量标准研究[J].中国药业,2011,20(6):30-31,2.
