新疆农业科学2011，Vol.48Issue(2)：247-251,5.
不同来源苹果茎沟病毒分离物部分序列分子特性研究
Study on the Molecular Characteristics of Partial Sequence from Different Sources Isolates of Apple stem Grooving Virus
摘要
Abstract
[Objective] In order to know the Apple Stem Grooving Virus infection status of pears in Xinjiang, and to identify gene sequences from obtaining virus . [Method] ASGV in pears from Xinjiang, Pyrus pyrifolia and Red pear were detected by biological and molecular methods and the gene sequences obtained were comparatively analyzed.[Result]The ASGV products with a expected size about 500 bp were amplified from 10 pear samples in Xinjiang, 2 samples of Pyrus pyrifolia and 3 Red pear samples from Yunnan province by RT - PCR. All these amplified products were cloned and sequenced . Comparison of these sequences showed that the CP gene from these isolates shared similarities ranging 88 .2 - 100％ ( nt) and 95 .6 - 100％ ( aa) , respectively. The phylogenetic tree was constructed based on amino acid of CP gene of detected ASCV isolates and reference isolates . Results showed that isolates KII- 3, KII - 5, KII - 7 of Korla pear, XII - 5 and XII - 6 of New pear no. 7 had lower genetic distances and clustered into a branch with a isolate KRL - 1 early reported from Korla pear, and another isolate P - 6 - 1 - 17 from Pyrus pyrifolia . In addition, isolates Kll - 10, Kll - 14 of Korla pear, Xll - 10 of New pear no. 7 was closer to YN - 2, YN - 4 of Red pear and they were clustered into another branch . [ Conclusion] Phylogenetic tree of cp gene nucleotide sequences displayed 8 ASGV isolates from Xinjiang and 3 isolates from Red pear have complex genetic variation phenomenon and in two different branches aeparately .关键词
苹果茎沟病毒/系统发育树/遗传变异分类
农业科技引用本文复制引用
黄妍妍,王国平,洪霓..不同来源苹果茎沟病毒分离物部分序列分子特性研究[J].新疆农业科学,2011,48(2):247-251,5.基金项目
国家梨产业技术体系(nycytx-29-08) （nycytx-29-08）
