中国油料作物学报2011，Vol.33Issue(2)：93-97,5.
油菜热休克蛋白基因HSP81.1启动子的克隆与功能验证
Cloning and function analysis of promoter of heat shock protein 81.1 from Brassica napus L.
摘要
Abstract
Promoter of temperature -induced heat shock protein (HSP) gene is used to control the exogenous gene expression not only to improve the expression accuracy, but also to enhance the safety of GM (genetically modified) crop. In present study, a HSP gene promoter from Brassica napus L. was cloned onto the upstream of GUS reporter gene in binary vector pBI121, in order to construct a binary expression vector pBI121 HSP GUS. The vector was then transformed into tobacco by Agrobacterium tumefaciens - mediated method. It was shown that T1 plants started to express the foreign GUS gene at 42℃ induction for 1h. The expression rate and amount of GUS gene varied at different growth stages and reached to a high level around the 50th day after seeding. Then the expression decreased gradually. The results indicated a potential of HSP promoter from rapeseed to control the expression of tobacco target gene under heat condition.关键词
热休克蛋白启动子/GUS报告基因/油菜/转基因烟草Key words
Heat shock promoter/ GUS reporter gene/ Brassica napus L./ Transgenic tobacco分类
农业科技引用本文复制引用
陈桂敏,吴刚,曹应龙,武玉花,肖玲,卢长明..油菜热休克蛋白基因HSP81.1启动子的克隆与功能验证[J].中国油料作物学报,2011,33(2):93-97,5.基金项目
国家863计划(2008AA10Z152) （2008AA10Z152）
