中国药房2011，Vol.22Issue(21)：1938-1940,3.
高效液相色谱荧光法测定家兔血浆中辣椒碱纳米乳的浓度及其药动学研究
Determination of Plasma Concentration of Capsaicin Nanoemulsions in Rabbits by HPLC-FLU and Study of Its Pharmacokinetics
张鹏威 1苏文琴 1张莉 2范开 3刘明生1
作者信息
- 1. 海南医学院药学院,海口市,571101
- 2. 武警医学院药剂教研室,天津市,300162
- 3. 天津天士力现代中药资源有限公司,天津市,300402
- 折叠
摘要
Abstract
OBJECTIVE: To develop the method for the content determination of capsaicin nanoemulsion in rabbit plasma and to study its pharmacokinetics.METHODS: 6 rabbits were randomly divided into two groups, then given single dose of capsaicin nanoemulsions or capsaicin solution 1 mg·kg-1 intravenously by double cycle crossover design, and after 3 min, 6 min, 9 min, 12 min, 15 min, 25 min, 40 min, 60 min, 90 min and 120 min, blood samples were collected.HPLC- FLU was established to determine the plasma concentration of capsaicin and the pharmacokinetic parameters were calculated.The determination was performed on Shimadzu VP-ODS C18 column with mobile phase consisted of methanol-water-phosphoric acid (85: 15: 0.1).The excitation wavelength was set at 280 nm and the detection wavelength was set at 310 nm.RESULTS: The linear range of capsaicin was 0.019 74～4.934 μg·mL-1(r=0.999 5) and the lowest detection limit was 4.9 ng·mL-1.The average recoveries were 90.4％～113.8％ (RSD≤10.1％ ).t1/2 of capsaicin nanoemulsion and capsaicin solution were (11.77 ± 2.32) min and (9.53 ± 2.19) min; AUC0～∞ were(5 311 ± 1 324.6) ng·min·mL-1 and(4 909.5± 1 110.4) ng·min·mL-1; cmax were 315.7 ng·mL-1 and 300.8 ng·mL-1 respectively.There was no significant difference.CONCLUSION: The method is simple, accurate and sensitive for the plasma concentration de-termination of capsaicin.Intravenous administration of nanoemulsion prepared by capsaicin doesn' t affect the pharmacokinetic parameter of it.关键词
辣椒碱/纳米乳/高效液相色谱荧光法/兔/药动学/血药浓度Key words
Capsaicin/ Nanoemulsions/ HPLC-FLU/ Rabbits/ Pharmacokinetics/ Plasma concentration分类
医药卫生引用本文复制引用
张鹏威,苏文琴,张莉,范开,刘明生..高效液相色谱荧光法测定家兔血浆中辣椒碱纳米乳的浓度及其药动学研究[J].中国药房,2011,22(21):1938-1940,3.
