临床小儿外科杂志2011，Vol.10Issue(2)：87-89,93,4.DOI:10.3969/j.issn.1671-6353.2011.02.003
肠外营养导致大鼠肝细胞炎症因子及受体基因表达的变化及临床意义
Parenteral Nutrition Leads to Alteration of Inflammation Cytokines and Receptors Gene Expression in the Rat
吴卫卫 1汪健 1黄顺根1
作者信息
- 1. 苏州大学附属儿童医院(江苏省苏州市,215003)
- 折叠
摘要
Abstract
Objective Total parenteral nutrition has been widely used during the past thirties years. It has been found to be an effective and relatively safe method for supplying energy and nutrients to patients with intestinal dysfunction, however, long-term TPN induces a high rate of liver disease in infants. The goal of this experiment was to further study the role of inflammation cytokines and receptors and the potential mechanism of TPN-induced hepatocyte injury by investigating the differential expresision of the inflammatory cytokine genes and receptors. Methods Twelve infant (22 ～28 days old ) male SD rats weighing 90 ～100 g were randomly divided into two groups (n =6). The control group received an oral diet, while the total parenteral nutrition group received continuous TPN infusion through a silastic catheter inserted in the right jugular vein. After 7 days part of the liver was taken for the examination of inflammatory cytokines and receptors gene expression with the oligo gene microarry to analyse the differential expression of the genes in the rats receiving TPN infusion. Results Compared with the control group, the 7-day TPN group was associated with an up-regulation of IL1r2IL -1β IL1rn IL - 18 TGF - α TGF - v1 TGF - β2 TGF - β3 TGF - β1il TNF-sf6 IFN - γbp Ccl4 Cyrl, and a down-regulation of Bmp2 XCRl FGF1O FGFr2 IL - 15 TGF -βr2 Scya11 FGF1 IL - 1Oα LTa Bambi Caspase1 Cxcl10 FGF16 FGF21 Frag1 TNFsf4. Conclusions The rats after TPN show immune suppression in the liver and a slow immune response. The up-regulation of IL - 1β , TGF - α and IL - 18 may be related to TPN-induced liver injury.关键词
胃肠外营养/全/肝细胞/炎症趋化因子类/大鼠引用本文复制引用
吴卫卫,汪健,黄顺根..肠外营养导致大鼠肝细胞炎症因子及受体基因表达的变化及临床意义[J].临床小儿外科杂志,2011,10(2):87-89,93,4.
